Calcitriol Started in the Donor, Expands the Population of CD4+CD25+ T Cells in Renal Transplant Recipients

M. R. Ardalan, H. Maljaei, Mohammadali Mohajel Shoja, A. R. Piri, H. T. Khosroshahi, H. Noshad, H. Argani

Research output: Contribution to journalArticle

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Abstract

Objectives: Alloreactive T cells recognize antigens via direct and indirect pathways. The competency of costimulatory molecules on antigen-presenting cells (APC) is important. An active form of vitamin D (1,25(OH)2D3, calcitriol) inhibits APC cell maturation and expression of costimulatory molecules. Herein we studied the immunosuppressive effects of calcitriol, which was started in the donors and continued in the kidney recipients. Methods: In this prospective study, candidates for living donor renal transplantation were randomly assigned into two groups: the treatment group were prescribed calcitriol (0.5 μg/day) started in the donor 6 days before donation and continued in recipient side for 6 months after transplantation. The control group received the conventional immunosuppressive regimen, namely, cyclosporine/mycophenolate mofetil and prednisolone. In each group, a recipient blood sample was obtained before and 6 months after transplantation. Diagnostic study of the T-cell markers-CD3, CD4, and CD25-were performed with a flow cytometery technique. Results: The mean values of CD3+CD4+CD25+ T cells in the treatment group (four women and five men; 40.8 ± 8.5 years) and the control group (four women and six men; 37.2 ± 10 years) were at 14.2 ± 4.2% and 15.4 ± 4.5% of total peripheral lymphocytes. Six months after transplantation, these percentages increased to 29 ± 6.3% in the treatment group and decreased to 12.1 ± 4.5% in the controls (P < .0001). No clinical rejection was detected in either group during the study period. Conclusion: Calcitriol started in the donors and continued in the kidney allograft recipients lead to expansion of CD4+CD25+ regulatory T cells in recipients. We speculated that costimulatory deficient APC for both direct and in-direct pathways may play a role.

Original languageEnglish (US)
Pages (from-to)951-953
Number of pages3
JournalTransplantation Proceedings
Volume39
Issue number4
DOIs
StatePublished - May 1 2007
Externally publishedYes

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Calcitriol
Antigen-Presenting Cells
Tissue Donors
T-Lymphocytes
Kidney
Transplantation
Immunosuppressive Agents
Population
Mycophenolic Acid
Control Groups
Living Donors
Regulatory T-Lymphocytes
Prednisolone
Vitamin D
Kidney Transplantation
Cyclosporine
Allografts
Therapeutics
Prospective Studies
Lymphocytes

ASJC Scopus subject areas

  • Surgery
  • Transplantation

Cite this

Ardalan, M. R., Maljaei, H., Mohajel Shoja, M., Piri, A. R., Khosroshahi, H. T., Noshad, H., & Argani, H. (2007). Calcitriol Started in the Donor, Expands the Population of CD4+CD25+ T Cells in Renal Transplant Recipients. Transplantation Proceedings, 39(4), 951-953. https://doi.org/10.1016/j.transproceed.2007.04.012

Calcitriol Started in the Donor, Expands the Population of CD4+CD25+ T Cells in Renal Transplant Recipients. / Ardalan, M. R.; Maljaei, H.; Mohajel Shoja, Mohammadali; Piri, A. R.; Khosroshahi, H. T.; Noshad, H.; Argani, H.

In: Transplantation Proceedings, Vol. 39, No. 4, 01.05.2007, p. 951-953.

Research output: Contribution to journalArticle

Ardalan, MR, Maljaei, H, Mohajel Shoja, M, Piri, AR, Khosroshahi, HT, Noshad, H & Argani, H 2007, 'Calcitriol Started in the Donor, Expands the Population of CD4+CD25+ T Cells in Renal Transplant Recipients', Transplantation Proceedings, vol. 39, no. 4, pp. 951-953. https://doi.org/10.1016/j.transproceed.2007.04.012
Ardalan, M. R. ; Maljaei, H. ; Mohajel Shoja, Mohammadali ; Piri, A. R. ; Khosroshahi, H. T. ; Noshad, H. ; Argani, H. / Calcitriol Started in the Donor, Expands the Population of CD4+CD25+ T Cells in Renal Transplant Recipients. In: Transplantation Proceedings. 2007 ; Vol. 39, No. 4. pp. 951-953.
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abstract = "Objectives: Alloreactive T cells recognize antigens via direct and indirect pathways. The competency of costimulatory molecules on antigen-presenting cells (APC) is important. An active form of vitamin D (1,25(OH)2D3, calcitriol) inhibits APC cell maturation and expression of costimulatory molecules. Herein we studied the immunosuppressive effects of calcitriol, which was started in the donors and continued in the kidney recipients. Methods: In this prospective study, candidates for living donor renal transplantation were randomly assigned into two groups: the treatment group were prescribed calcitriol (0.5 μg/day) started in the donor 6 days before donation and continued in recipient side for 6 months after transplantation. The control group received the conventional immunosuppressive regimen, namely, cyclosporine/mycophenolate mofetil and prednisolone. In each group, a recipient blood sample was obtained before and 6 months after transplantation. Diagnostic study of the T-cell markers-CD3, CD4, and CD25-were performed with a flow cytometery technique. Results: The mean values of CD3+CD4+CD25+ T cells in the treatment group (four women and five men; 40.8 ± 8.5 years) and the control group (four women and six men; 37.2 ± 10 years) were at 14.2 ± 4.2{\%} and 15.4 ± 4.5{\%} of total peripheral lymphocytes. Six months after transplantation, these percentages increased to 29 ± 6.3{\%} in the treatment group and decreased to 12.1 ± 4.5{\%} in the controls (P < .0001). No clinical rejection was detected in either group during the study period. Conclusion: Calcitriol started in the donors and continued in the kidney allograft recipients lead to expansion of CD4+CD25+ regulatory T cells in recipients. We speculated that costimulatory deficient APC for both direct and in-direct pathways may play a role.",
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AU - Ardalan, M. R.

AU - Maljaei, H.

AU - Mohajel Shoja, Mohammadali

AU - Piri, A. R.

AU - Khosroshahi, H. T.

AU - Noshad, H.

AU - Argani, H.

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