Calcium phosphate crystal formation in Escherichia coli from human urine: An in vitro study

M. S. Cohen, C. P. Davis, E. W. Czerwinski, M. M. Warren

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Escherichia coli with no demonstrable urease activity was inoculated into filter sterilized urine obtained from a healthy volunteer subject with no history of stone disease and then incubated at 37C. Bacteria were recovered at intervals between 1 and 10 days. Urinary pH was stable as compared to control urines and spontaneous crystal precipitation was not noted in controls. Recovered organisms were analyzed by x-ray powder diffractometry. An uncharacterized mineral phase (UMP) was first evident after 6 days. Calcium phosphate in the form of brushite and hydroxyapatite was apparent at 7 and 10 days respectively. This suggests a role for bacteria in calcium phosphate crystal formation in urine apart from urease activity and may contribute to the calcium phosphate component of urinary calculi.

Original languageEnglish (US)
Pages (from-to)184-185
Number of pages2
JournalJournal of Urology
Volume127
Issue number1
StatePublished - 1982

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Urease
Urine
Escherichia coli
Healthy Volunteers
Bacteria
Urinary Calculi
Durapatite
Powders
Minerals
X-Rays
In Vitro Techniques
calcium phosphate
dibasic calcium phosphate dihydrate

ASJC Scopus subject areas

  • Urology

Cite this

Cohen, M. S., Davis, C. P., Czerwinski, E. W., & Warren, M. M. (1982). Calcium phosphate crystal formation in Escherichia coli from human urine: An in vitro study. Journal of Urology, 127(1), 184-185.

Calcium phosphate crystal formation in Escherichia coli from human urine : An in vitro study. / Cohen, M. S.; Davis, C. P.; Czerwinski, E. W.; Warren, M. M.

In: Journal of Urology, Vol. 127, No. 1, 1982, p. 184-185.

Research output: Contribution to journalArticle

Cohen, MS, Davis, CP, Czerwinski, EW & Warren, MM 1982, 'Calcium phosphate crystal formation in Escherichia coli from human urine: An in vitro study', Journal of Urology, vol. 127, no. 1, pp. 184-185.
Cohen, M. S. ; Davis, C. P. ; Czerwinski, E. W. ; Warren, M. M. / Calcium phosphate crystal formation in Escherichia coli from human urine : An in vitro study. In: Journal of Urology. 1982 ; Vol. 127, No. 1. pp. 184-185.
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