Calcium phosphate crystal formation in Escherichia coli from human urine: An in vitro study

M. S. Cohen; C. P. Davis; E. W. Czerwinski; M. M. Warren

doi:10.1016/S0022-5347(17)53658-7

Calcium phosphate crystal formation in Escherichia coli from human urine: An in vitro study

M. S. Cohen, C. P. Davis, E. W. Czerwinski, M. M. Warren

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Abstract

Escherichia coli with no demonstrable urease activity was inoculated into filter sterilized urine obtained from a healthy volunteer subject with no history of stone disease and then incubated at 37C. Bacteria were recovered at intervals between 1 and 10 days. Urinary pH was stable as compared to control urines and spontaneous crystal precipitation was not noted in controls. Recovered organisms were analyzed by x-ray powder diffractometry. An uncharacterized mineral phase (UMP) was first evident after 6 days. Calcium phosphate in the form of brushite and hydroxyapatite was apparent at 7 and 10 days respectively. This suggests a role for bacteria in calcium phosphate crystal formation in urine apart from urease activity and may contribute to the calcium phosphate component of urinary calculi.

Original language	English (US)
Pages (from-to)	184-185
Number of pages	2
Journal	Journal of Urology
Volume	127
Issue number	1
DOIs	https://doi.org/10.1016/S0022-5347(17)53658-7
State	Published - Jan 1 1982

ASJC Scopus subject areas

Urology

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AU - Czerwinski, E. W.

AU - Warren, M. M.

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AB - Escherichia coli with no demonstrable urease activity was inoculated into filter sterilized urine obtained from a healthy volunteer subject with no history of stone disease and then incubated at 37C. Bacteria were recovered at intervals between 1 and 10 days. Urinary pH was stable as compared to control urines and spontaneous crystal precipitation was not noted in controls. Recovered organisms were analyzed by x-ray powder diffractometry. An uncharacterized mineral phase (UMP) was first evident after 6 days. Calcium phosphate in the form of brushite and hydroxyapatite was apparent at 7 and 10 days respectively. This suggests a role for bacteria in calcium phosphate crystal formation in urine apart from urease activity and may contribute to the calcium phosphate component of urinary calculi.

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Calcium phosphate crystal formation in Escherichia coli from human urine: An in vitro study

Abstract

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