Canavan disease: Biochemical and molecular studies

Reuben Matalon, R. Kaul, K. Michals

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Deficiency of the enzyme aspartoacylase and the accumulation of N-acetylaspartic acid lead to a severe leukodystrophy and spongy degeneration of the brain, Canavan disease (McKusick 271900). Since our discovery in 1988 of the defect in Canavan disease, 144 patients with Canavan disease have been diagnosed in our laboratory. Most of these children are of Ashkenazi Jewish extraction. The level of enzyme activity can be used for carrier testing. Prenatal diagnosis has been difficult using the enzyme assay owing to the low activity of aspartoacylase in cultured chorionic villus samples or amniocytes. The determination of N-acetylaspartic acid in the amniotic fluid is another parameter for diagnosis; however, the levels may not always be elevated. Bovine and human aspartoacylase have been purified in our laboratory. Bovine and human cDNA and genomic clones have been isolated and six exons have been localized. This information is being used for the study of Canavan disease at the molecular level.

Original languageEnglish (US)
Pages (from-to)744-752
Number of pages9
JournalJournal of Inherited Metabolic Disease
Volume16
Issue number4
DOIs
StatePublished - Jul 1993
Externally publishedYes

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Canavan Disease
Chorionic Villi
Enzyme Assays
Brain Diseases
Enzymes
Amniotic Fluid
Prenatal Diagnosis
Exons
Complementary DNA
Clone Cells

ASJC Scopus subject areas

  • Genetics(clinical)
  • Genetics
  • Endocrinology

Cite this

Canavan disease : Biochemical and molecular studies. / Matalon, Reuben; Kaul, R.; Michals, K.

In: Journal of Inherited Metabolic Disease, Vol. 16, No. 4, 07.1993, p. 744-752.

Research output: Contribution to journalArticle

Matalon, Reuben ; Kaul, R. ; Michals, K. / Canavan disease : Biochemical and molecular studies. In: Journal of Inherited Metabolic Disease. 1993 ; Vol. 16, No. 4. pp. 744-752.
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