Candidatus Rickettsia senegalensis in Cat Fleas (Siphonaptera: Pulicidae) Collected from Dogs and Cats in Cauca, Colombia

Paola Betancourt-Ruiz, Heidy C. Martínez-Díaz, Juliana Gil-Mora, Catalina Ospina, Luz Adriana Olaya-M, Efraín Benavides, Eliana Bolaños, Claudia Cuervo, Lucas Blanton, Marylin Hidalgo

    Research output: Contribution to journalArticle

    Abstract

    Rickettsia typhi and Rickettsia felis (Rickettsiales: Rickettsiaceae) are flea-transmitted pathogens. They are important causes of acute febrile illness throughout the world. We, therefore, sought to identify the rickettsial species present in the fleas of dogs and cats in the department of Cauca, Colombia. In this study, we collected 1,242 fleas from 132 dogs and 43 fleas from 11 cats. All fleas were morphologically identified as Ctenocephalides felis (Bouché) adults and organized in pools for DNA extraction (234 pools from dogs and 11 from cats). The gltA gene from rickettsiae was targeted for screening amplification using conventional PCR. In total, 144 of the 245 pools (58.7%) were positive. The positive samples were then processed for the amplification of the 17kDa antigen gene (144/144; 100% positive) and sca5 gene (140/144; 97.2% positive). In addition, restriction enzyme length polymorphism analysis using NlaIV on the amplified product of the sca5 gene demonstrated several organisms: 21/140 (15%) were R. felis, 118/140 (84.3%) were Rickettsia asemboensis, and 1/140 (0.7%) were Candidatus Rickettsia senegalensis. Subsequent sequencing confirmed Candidatus Rickettsia senegalensis in C. felis collected from dogs the first reported from Colombia.

    Original languageEnglish (US)
    Pages (from-to)382-387
    Number of pages6
    JournalJournal of medical entomology
    Volume57
    Issue number2
    DOIs
    StatePublished - Feb 27 2020

    Keywords

    • Ctenocephalides felis
    • flea-borne rickettsioses
    • Rickettsia asemboensis
    • Rickettsia senegalensis
    • Rickettsia spp

    ASJC Scopus subject areas

    • Parasitology
    • veterinary(all)
    • Insect Science
    • Infectious Diseases

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  • Cite this

    Betancourt-Ruiz, P., Martínez-Díaz, H. C., Gil-Mora, J., Ospina, C., Olaya-M, L. A., Benavides, E., Bolaños, E., Cuervo, C., Blanton, L., & Hidalgo, M. (2020). Candidatus Rickettsia senegalensis in Cat Fleas (Siphonaptera: Pulicidae) Collected from Dogs and Cats in Cauca, Colombia. Journal of medical entomology, 57(2), 382-387. https://doi.org/10.1093/jme/tjz177