Canine cyclin T1 rescues equine infectious anemia virus tat trans- activation in human cells

Todd R. Albrecht, Lars H. Lund, Mariano Garcia-Blanco

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Human immunodeficiency virus-1 Tat protein and human Cyclin T1 mediate transcriptional activation by enhancing the elongation efficiency of RNA polymerase II. Activation of transcription of the related equine infectious anemia virus (EIAV) requires a similar protein known as eTat, which does not function in human cells. Expression of equine Cyclin T1 in human cells rescues eTat function, suggesting a general mechanism of transcription activation among lentiviruses. Here we present the cloning of Cyclin T1 from canine D17 osteosarcoma cells, which support EIAV transactivation, and show that canine Cyclin T1 confers eTat transactivation to human cells. A two- amino-acid change, from 79-proline-glycine-80 to 79-histidine-arginine-80, confers on the human Cyclin T1 the ability to cooperate with eTat in transcriptional activation. These findings suggested that the regions of Cyclin T1 that interact with lentiviral Tat proteins and TAR RNA elements form an extended domain, which very likely has a conserved fold. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)7-11
Number of pages5
JournalVirology
Volume268
Issue number1
DOIs
StatePublished - Mar 1 2000
Externally publishedYes

Fingerprint

Cyclin T
Equine infectious anemia virus
Transcriptional Activation
Canidae
tat Gene Products
Lentivirus
RNA Polymerase II
Osteosarcoma
Histidine
Proline
Glycine
Horses
Arginine
HIV-1
Organism Cloning
RNA
Amino Acids

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Canine cyclin T1 rescues equine infectious anemia virus tat trans- activation in human cells. / Albrecht, Todd R.; Lund, Lars H.; Garcia-Blanco, Mariano.

In: Virology, Vol. 268, No. 1, 01.03.2000, p. 7-11.

Research output: Contribution to journalArticle

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