Canine haptoglobin: A unique haptoglobin subunit arrangement

Alexander Kurosky, Regine E. Hay, Barbara H. Bowman

    Research output: Contribution to journalArticle

    16 Citations (Scopus)

    Abstract

    1. 1. Isolated canine haptoglobin behaved identically to the α2β2 structure typical of human haptoglobin type 1-1 on alkaline polyacrylamide gel electrophoresis and on gel filtration. 2. 2. In the presence of urea or sodium dodecyl sulphate canine haptoglobin dissociated into αβ subunits that separated into α and β chains after reduction with 2-mercaptoethanol. 3. 3. Compositional analysis identified one less half-cystine in canine α chain when compared to human α1 chain. 4. 4. These results provide evidence that there is no inter α chain disulphide in canine haptoglobin comparable to the α120-α120 disulphide in human haptoglobin that links the two αβ subunits.

    Original languageEnglish (US)
    Pages (from-to)339-344
    Number of pages6
    JournalComparative Biochemistry and Physiology -- Part B: Biochemistry and
    Volume62
    Issue number4
    DOIs
    StatePublished - 1979

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    Haptoglobins
    Canidae
    Disulfides
    Mercaptoethanol
    Electrophoresis
    Sodium Dodecyl Sulfate
    Cysteine
    Urea
    Gels
    Gel Chromatography
    Polyacrylamide Gel Electrophoresis
    human HP protein

    ASJC Scopus subject areas

    • Medicine(all)

    Cite this

    Canine haptoglobin : A unique haptoglobin subunit arrangement. / Kurosky, Alexander; Hay, Regine E.; Bowman, Barbara H.

    In: Comparative Biochemistry and Physiology -- Part B: Biochemistry and, Vol. 62, No. 4, 1979, p. 339-344.

    Research output: Contribution to journalArticle

    Kurosky, Alexander ; Hay, Regine E. ; Bowman, Barbara H. / Canine haptoglobin : A unique haptoglobin subunit arrangement. In: Comparative Biochemistry and Physiology -- Part B: Biochemistry and. 1979 ; Vol. 62, No. 4. pp. 339-344.
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