@article{58156273b9b645faa590911142c314c7,
title = "Canine haptoglobin: A unique haptoglobin subunit arrangement",
abstract = "1. 1. Isolated canine haptoglobin behaved identically to the α2β2 structure typical of human haptoglobin type 1-1 on alkaline polyacrylamide gel electrophoresis and on gel filtration. 2. 2. In the presence of urea or sodium dodecyl sulphate canine haptoglobin dissociated into αβ subunits that separated into α and β chains after reduction with 2-mercaptoethanol. 3. 3. Compositional analysis identified one less half-cystine in canine α chain when compared to human α1 chain. 4. 4. These results provide evidence that there is no inter α chain disulphide in canine haptoglobin comparable to the α120-α120 disulphide in human haptoglobin that links the two αβ subunits.",
author = "Alexander Kurosky and Hay, {Regine E.} and Bowman, {Barbara H.}",
note = "Funding Information: In all species investigated that possess nonpoly-meric Hp, the functional molecule is a tetrachain structure comprised of two ~t and two fl chains that are covalently linked by interchain disulphide bonds (Smithies et al., 1962a; Malchy & Dixon, 1973a; Malchy et al., 1973; Kurosky et al., 1976a; Shim et al., 1971; Lockhart et al., 1972; Shim et al., 1975; Kong & Shim, 1974). A single disulphide bridge attaches the ~t and fl chains to form an 0~fl subunit in human Hp 1-1. Two such subunits are combined by an ct120-ct120 disulphide bond (Malchy & Dixon, 1973a; Kurosky et al., 1976a). The structure of human * This investigation was supported by grant HD 03321 from the National Institute of Child Health and Human Development. t To whom correspondence should be addressed. § Abbreviations: Hp, haptoglobin (intact); hp, haptoglobin (chains); Hb, hemoglobin; Pth, phenylthiohydantoin; SDS, sodium dodecyl sulphate; EDTA, ethylenediaminetetracetic acid. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.",
year = "1979",
doi = "10.1016/0305-0491(79)90100-7",
language = "English (US)",
volume = "62",
pages = "339--344",
journal = "Comparative Biochemistry and Physiology -- Part B: Biochemistry and",
issn = "0305-0491",
publisher = "Elsevier BV",
number = "4",
}