CAPA-gene products in the haematophagous sandfly Phlebotomus papatasi (Scopoli)-vector for leishmaniasis disease

Reinhard Predel, Susanne Neupert, William Russell, Frank Hauser, David H. Russell, Andrew Li, Ronald J. Nachman

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Sandflies (Phlebotominae, Nematocera, Diptera) are responsible for transmission of leishmaniasis and other protozoan-borne diseases in humans, and these insects depend on the regulation of water balance to cope with the sudden and enormous intake of blood over a very short time period. The sandfly inventory of neuropeptides, including those that regulate diuretic processes, is completely unknown. Direct MALDI-TOF/TOF mass spectrometric analysis of dissected ganglia of Phlebotomus papatasi, combined with a data-mining of sandfly genome 'contigs', was used to identify native CAPA-peptides, a peptide class associated with the regulation of diuresis in other hematophagous insects. The CAPA-peptides identified in this study include two CAPA-PVKs, differentially processed CAPA-PK, and an additional CAPA precursor peptide. The mass spectrometric analysis of different parts of the neuroendocrine system of the sandfly indicate that it represents the first insect which accumulates CAPA-PVKs exclusively in hormone release sites of abdominal ganglia and CAPA-PK (nearly) exclusively in the corpora cardiaca. Additionally, sandflies feature the smallest abdominal ganglia (∼35 μm) where CAPA-peptides could be detected so far. The small size of the abdominal ganglia does not appear to affect the development of the median neurosecretory system as it obviously does in another comparably small insect species, Nasonia vitripennis, in which no capa-gene expression was found. Rather, immunocytochemical analyses confirm that the general architecture in sandflies appears identical to that of much larger mosquitoes.

Original languageEnglish (US)
Pages (from-to)2-7
Number of pages6
JournalPeptides
Volume41
DOIs
StatePublished - Mar 2013
Externally publishedYes

Fingerprint

Phlebotomus
Disease Vectors
Psychodidae
Leishmaniasis
Genes
Peptides
Ganglia
Insects
Neurosecretory Systems
Corpora Allata
Neuropeptides
Diuretics
Gene expression
Data mining
Data Mining
Diuresis
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Blood
Culicidae
Hormones

Keywords

  • Capa-gene
  • Corazonin
  • Differential processing
  • Insect neuropeptides
  • MALDI-TOF
  • Peptidomics
  • Periviscerokinins
  • Phlebotomus papatasi
  • Psychodidae

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Cellular and Molecular Neuroscience

Cite this

CAPA-gene products in the haematophagous sandfly Phlebotomus papatasi (Scopoli)-vector for leishmaniasis disease. / Predel, Reinhard; Neupert, Susanne; Russell, William; Hauser, Frank; Russell, David H.; Li, Andrew; Nachman, Ronald J.

In: Peptides, Vol. 41, 03.2013, p. 2-7.

Research output: Contribution to journalArticle

Predel, Reinhard ; Neupert, Susanne ; Russell, William ; Hauser, Frank ; Russell, David H. ; Li, Andrew ; Nachman, Ronald J. / CAPA-gene products in the haematophagous sandfly Phlebotomus papatasi (Scopoli)-vector for leishmaniasis disease. In: Peptides. 2013 ; Vol. 41. pp. 2-7.
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