Capillary electrophoresis and its application in the clinical laboratory

John R. Petersen, Anthony Okorodudu, Amin Mohammad, Deborah A. Payne

Research output: Contribution to journalArticle

99 Citations (Scopus)

Abstract

Over the past 10 years, capillary electrophoresis (CE) is an analytical tool that has shown great promise in replacing many conventional clinical laboratory methods, especially electrophoresis and high performance liquid chromatography (HPLC). The main attraction of CE was that it was fast, used small amounts of sample and reagents, and was extremely versatile, being able to separate large and small analytes, both neutral and charged. Because of this versatility, numerous methods for clinically relevant analytes have been developed. However, with the exception of the molecular diagnostic and forensic laboratories CE has not had a major impact. A possible reason is that CE is still perceived as requiring above-average technical expertise, precluding its use in a laboratory workforce that is less technically adept. With the introduction of multicapillary instruments that are more automated, less technique-dependent, in addition to the availability of commercial and cost effective test kit methods, CE may yet be accepted as a instrument routinely used in the clinical laboratories. Thus, this review will focus on the areas where CE shows the most potential to have the greatest impact on the clinical laboratory. These include analysis of proteins found in serum, urine, CSF and body fluids, immunosubstraction electrophoresis, hemoglobin variants, lipoproteins, carbohydrate-deficient transferrin (CDT), forensic and therapeutic drug screening, and molecular diagnostics.

Original languageEnglish (US)
Pages (from-to)1-30
Number of pages30
JournalClinica Chimica Acta
Volume330
Issue number1-2
DOIs
StatePublished - Apr 2003

Fingerprint

Clinical laboratories
Capillary electrophoresis
Capillary Electrophoresis
Molecular Pathology
Electrophoresis
Professional Competence
Preclinical Drug Evaluations
Body fluids
High performance liquid chromatography
Body Fluids
Lipoproteins
Screening
Hemoglobins
High Pressure Liquid Chromatography
Availability
Urine
Costs and Cost Analysis
Serum
Pharmaceutical Preparations
Costs

Keywords

  • Capillary electrophoresis
  • Clinical applications
  • Clinical laboratory
  • Molecular diagnostics

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry

Cite this

Capillary electrophoresis and its application in the clinical laboratory. / Petersen, John R.; Okorodudu, Anthony; Mohammad, Amin; Payne, Deborah A.

In: Clinica Chimica Acta, Vol. 330, No. 1-2, 04.2003, p. 1-30.

Research output: Contribution to journalArticle

Petersen, John R. ; Okorodudu, Anthony ; Mohammad, Amin ; Payne, Deborah A. / Capillary electrophoresis and its application in the clinical laboratory. In: Clinica Chimica Acta. 2003 ; Vol. 330, No. 1-2. pp. 1-30.
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