Capsid protein structure in Zika virus reveals the flavivirus assembly process

Ter Yong Tan, Guntur Fibriansah, Victor A. Kostyuchenko, Thiam Seng Ng, Xin Xiang Lim, Shuijun Zhang, Xin Ni Lim, Jiaqi Wang, Jian Shi, Marc C. Morais, Davide Corti, Shee Mei Lok

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Structures of flavivirus (dengue virus and Zika virus) particles are known to near-atomic resolution and show detailed structure and arrangement of their surface proteins (E and prM in immature virus or M in mature virus). By contrast, the arrangement of the capsid proteins:RNA complex, which forms the core of the particle, is poorly understood, likely due to inherent dynamics. Here, we stabilize immature Zika virus via an antibody that binds across the E and prM proteins, resulting in a subnanometer resolution structure of capsid proteins within the virus particle. Fitting of the capsid protein into densities shows the presence of a helix previously thought to be removed via proteolysis. This structure illuminates capsid protein quaternary organization, including its orientation relative to the lipid membrane and the genomic RNA, and its interactions with the transmembrane regions of the surface proteins. Results show the capsid protein plays a central role in the flavivirus assembly process.

Original languageEnglish (US)
Article number895
JournalNature communications
Volume11
Issue number1
DOIs
StatePublished - Dec 1 2020

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

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    Tan, T. Y., Fibriansah, G., Kostyuchenko, V. A., Ng, T. S., Lim, X. X., Zhang, S., Lim, X. N., Wang, J., Shi, J., Morais, M. C., Corti, D., & Lok, S. M. (2020). Capsid protein structure in Zika virus reveals the flavivirus assembly process. Nature communications, 11(1), [895]. https://doi.org/10.1038/s41467-020-14647-9