Capsid Structure and Its Stability at the Late Stages of Bacteriophage SPP1 Assembly

Helen E. White, Michael Sherman, Sandrine Brasilès, Eric Jacquet, Philippa Seavers, Paulo Tavares, Elena V. Orlova

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The structure of the bacteriophage SPP1 capsid was determined at subnanometer resolution by cryo-electron microscopy and single-particle analysis. The icosahedral capsid is composed of the major capsid protein gp13 and the auxiliary protein gp12, which are organized in a T=7 lattice. DNA is arranged in layers with a distance of~24.5 Å. gp12 forms spikes that are anchored at the center of gp13 hexamers. In a gp12-deficient mutant, the centers of hexamers are closed by loops of gp13 coming together to protect the SPP1 genome from the outside environment. The HK97-like fold was used to build a pseudoatomic model of gp13. Its structural organization remains unchanged upon tail binding and following DNA release. gp13 exhibits enhanced thermostability in the DNA-filled capsid. A remarkable convergence between the thermostability of the capsid and those of the other virion components was found, revealing that the overall architecture of the SPP1 infectious particle coevolved toward high robustness.

Original languageEnglish (US)
Pages (from-to)6768-6777
Number of pages10
JournalJournal of Virology
Volume86
Issue number12
DOIs
StatePublished - Jul 2012

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capsid
Capsid
Bacteriophages
thermal stability
DNA
Cryoelectron Microscopy
Capsid Proteins
coat proteins
virion
Virion
bacteriophages
tail
Genome
mutants
genome
virus assembly
Proteins
proteins

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

White, H. E., Sherman, M., Brasilès, S., Jacquet, E., Seavers, P., Tavares, P., & Orlova, E. V. (2012). Capsid Structure and Its Stability at the Late Stages of Bacteriophage SPP1 Assembly. Journal of Virology, 86(12), 6768-6777. https://doi.org/10.1128/JVI.00412-12

Capsid Structure and Its Stability at the Late Stages of Bacteriophage SPP1 Assembly. / White, Helen E.; Sherman, Michael; Brasilès, Sandrine; Jacquet, Eric; Seavers, Philippa; Tavares, Paulo; Orlova, Elena V.

In: Journal of Virology, Vol. 86, No. 12, 07.2012, p. 6768-6777.

Research output: Contribution to journalArticle

White, HE, Sherman, M, Brasilès, S, Jacquet, E, Seavers, P, Tavares, P & Orlova, EV 2012, 'Capsid Structure and Its Stability at the Late Stages of Bacteriophage SPP1 Assembly', Journal of Virology, vol. 86, no. 12, pp. 6768-6777. https://doi.org/10.1128/JVI.00412-12
White, Helen E. ; Sherman, Michael ; Brasilès, Sandrine ; Jacquet, Eric ; Seavers, Philippa ; Tavares, Paulo ; Orlova, Elena V. / Capsid Structure and Its Stability at the Late Stages of Bacteriophage SPP1 Assembly. In: Journal of Virology. 2012 ; Vol. 86, No. 12. pp. 6768-6777.
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