Carbon dioxide is a powerful inducer of monokaryotic hyphae and spore development in cryptococcus gattii and carbonic anhydrase activity is dispensable in this dimorphic transition

Ping Ren, Vishnu Chaturvedi, Sudha Chaturvedi

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Cryptococcus gattii is unique among human pathogenic fungi with specialized ecological niche on trees. Since leaves concentrate CO2, we investigated the role of this gaseous molecule in C. gattii biology and virulence. We focused on the genetic analyses of b-carbonic anhydrase (β-CA) encoded by C. gattii CAN1 and CAN2 as later is critical for CO2 sensing in a closely related pathogen C. neoformans. High CO2 conditions induced robust development of monokaryotic hyphae and spores in C. gattii. Conversely, high CO2 completely repressed hyphae development in sexual mating. Both CAN1 and CAN2 were dispensable for CO2 induced morphogenetic transitions. However, C. gattii CAN2 was essential for growth in ambient air similar to its reported role in C. neoformans. Both can1 and can2 mutants retained full pathogenic potential in vitro and in vivo. These results provide insight into C. gattii adaptation for arboreal growth and production of infectious propagules by b-CA independent mechanism(s).

Original languageEnglish (US)
Article numbere113147
JournalPLoS One
Volume9
Issue number12
DOIs
StatePublished - Oct 22 2014
Externally publishedYes

Fingerprint

Cryptococcus gattii
Carbonic Anhydrases
Hyphae
carbonate dehydratase
Spores
Carbon Dioxide
hyphae
spores
carbon dioxide
Pathogens
Fungi
animal pathogenic fungi
Molecules
Carbonic Anhydrase II
Air
Sexual Development
Growth
sexual development
Virulence
tree growth

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Carbon dioxide is a powerful inducer of monokaryotic hyphae and spore development in cryptococcus gattii and carbonic anhydrase activity is dispensable in this dimorphic transition. / Ren, Ping; Chaturvedi, Vishnu; Chaturvedi, Sudha.

In: PLoS One, Vol. 9, No. 12, e113147, 22.10.2014.

Research output: Contribution to journalArticle

@article{8150e73128f9488698d6cee09245d8c8,
title = "Carbon dioxide is a powerful inducer of monokaryotic hyphae and spore development in cryptococcus gattii and carbonic anhydrase activity is dispensable in this dimorphic transition",
abstract = "Cryptococcus gattii is unique among human pathogenic fungi with specialized ecological niche on trees. Since leaves concentrate CO2, we investigated the role of this gaseous molecule in C. gattii biology and virulence. We focused on the genetic analyses of b-carbonic anhydrase (β-CA) encoded by C. gattii CAN1 and CAN2 as later is critical for CO2 sensing in a closely related pathogen C. neoformans. High CO2 conditions induced robust development of monokaryotic hyphae and spores in C. gattii. Conversely, high CO2 completely repressed hyphae development in sexual mating. Both CAN1 and CAN2 were dispensable for CO2 induced morphogenetic transitions. However, C. gattii CAN2 was essential for growth in ambient air similar to its reported role in C. neoformans. Both can1 and can2 mutants retained full pathogenic potential in vitro and in vivo. These results provide insight into C. gattii adaptation for arboreal growth and production of infectious propagules by b-CA independent mechanism(s).",
author = "Ping Ren and Vishnu Chaturvedi and Sudha Chaturvedi",
year = "2014",
month = "10",
day = "22",
doi = "10.1371/journal.pone.0113147",
language = "English (US)",
volume = "9",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "12",

}

TY - JOUR

T1 - Carbon dioxide is a powerful inducer of monokaryotic hyphae and spore development in cryptococcus gattii and carbonic anhydrase activity is dispensable in this dimorphic transition

AU - Ren, Ping

AU - Chaturvedi, Vishnu

AU - Chaturvedi, Sudha

PY - 2014/10/22

Y1 - 2014/10/22

N2 - Cryptococcus gattii is unique among human pathogenic fungi with specialized ecological niche on trees. Since leaves concentrate CO2, we investigated the role of this gaseous molecule in C. gattii biology and virulence. We focused on the genetic analyses of b-carbonic anhydrase (β-CA) encoded by C. gattii CAN1 and CAN2 as later is critical for CO2 sensing in a closely related pathogen C. neoformans. High CO2 conditions induced robust development of monokaryotic hyphae and spores in C. gattii. Conversely, high CO2 completely repressed hyphae development in sexual mating. Both CAN1 and CAN2 were dispensable for CO2 induced morphogenetic transitions. However, C. gattii CAN2 was essential for growth in ambient air similar to its reported role in C. neoformans. Both can1 and can2 mutants retained full pathogenic potential in vitro and in vivo. These results provide insight into C. gattii adaptation for arboreal growth and production of infectious propagules by b-CA independent mechanism(s).

AB - Cryptococcus gattii is unique among human pathogenic fungi with specialized ecological niche on trees. Since leaves concentrate CO2, we investigated the role of this gaseous molecule in C. gattii biology and virulence. We focused on the genetic analyses of b-carbonic anhydrase (β-CA) encoded by C. gattii CAN1 and CAN2 as later is critical for CO2 sensing in a closely related pathogen C. neoformans. High CO2 conditions induced robust development of monokaryotic hyphae and spores in C. gattii. Conversely, high CO2 completely repressed hyphae development in sexual mating. Both CAN1 and CAN2 were dispensable for CO2 induced morphogenetic transitions. However, C. gattii CAN2 was essential for growth in ambient air similar to its reported role in C. neoformans. Both can1 and can2 mutants retained full pathogenic potential in vitro and in vivo. These results provide insight into C. gattii adaptation for arboreal growth and production of infectious propagules by b-CA independent mechanism(s).

UR - http://www.scopus.com/inward/record.url?scp=84916898905&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84916898905&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0113147

DO - 10.1371/journal.pone.0113147

M3 - Article

C2 - 25478697

AN - SCOPUS:84916898905

VL - 9

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 12

M1 - e113147

ER -