Carbonylation of ER chaperone proteins in aged mouse liver

Jeffrey P. Rabek, William H. Boylston, John Papaconstantinou

Research output: Contribution to journalArticle

112 Citations (Scopus)

Abstract

Progressive accumulation of oxidative damage to macromolecules in aged tissues is thought to contribute to the decline in tissue function characteristic of the aged phenotype. Mitochondria are a major intracellular source of reactive oxygen species (ROS); however, other organelles are also endogenous sources of oxyradicals and oxidants, which can damage macromolecules. We, therefore, sought to examine the relationship between aging and oxidative damage to ER resident proteins, which exist in a strongly oxidizing environment necessary for disulfide bond formation. In these studies, we have fractionated young and aged liver homogenates, resolved the proteins by 2D gel electrophoresis, assayed for oxidative damage as indicated by protein carbonylation, and identified BiP/Grp78, protein disulfide isomerase (PDI), and calreticulin as exhibiting an age-associated increase in oxidative damage. Increased carbonylation of these key proteins in aged liver suggests an age-associated impairment in protein folding, disulfide crosslinking, and glycosylation in the aged mouse liver.

Original languageEnglish (US)
Pages (from-to)566-572
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume305
Issue number3
DOIs
StatePublished - Jun 6 2003

Fingerprint

Carbonylation
Protein Carbonylation
Liver
Disulfides
Macromolecules
Calreticulin
Protein Disulfide-Isomerases
Proteins
Protein Folding
Electrophoresis, Gel, Two-Dimensional
Tissue
Glycosylation
Protein folding
Oxidants
Organelles
Mitochondria
Reactive Oxygen Species
Electrophoresis
Crosslinking
Phenotype

Keywords

  • Aging
  • Carbonylation
  • Chaperone proteins
  • Endoplasmic reticulum
  • Oxidative damage

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Carbonylation of ER chaperone proteins in aged mouse liver. / Rabek, Jeffrey P.; Boylston, William H.; Papaconstantinou, John.

In: Biochemical and Biophysical Research Communications, Vol. 305, No. 3, 06.06.2003, p. 566-572.

Research output: Contribution to journalArticle

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