Carboxyhemoglobin formation following smoke inhalation injury in sheep is interrelated with pulmonary shunt fraction

Martin Westphal; Naoki Morita; Perenlei Enkhbaatar; Kazunori Murakami; Lillian Traber; Daniel Lee Traber

doi:10.1016/j.bbrc.2003.10.063

Carboxyhemoglobin formation following smoke inhalation injury in sheep is interrelated with pulmonary shunt fraction

Martin Westphal, Naoki Morita, Perenlei Enkhbaatar, Kazunori Murakami, Lillian Traber, Daniel Lee Traber

Anesthesiology

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

Carboxyhemoglobin (COHb) formation is triggered by the inducible isoform of heme oxygenase (HO-1) catalyzing carbon monoxide (CO) production through breakdown of heme molecules, exposure to CO or both. In the setting of CO poisoning, COHb is regarded as a reliable marker characterizing both severity of injury and efficacy of treatment strategies. This study was designed as a prospective laboratory experiment to elucidate potential interdependencies between COHb generation, oxygenation, and pulmonary shunt fraction (Qs/Qt) in an ovine model of smoke inhalation injury. Chronically instrumented ewes (n=15) were repeatedly subjected to cotton smoke (4×12 breaths) according to an established protocol. This approach resulted in a progressive increase in COHb formation that was interrelated with the degree of Qs/Qt (P<0.001) and inversely correlated with both arterial and mixed venous HbO₂ saturation (r=-0.96 and -0.93). Although the arteriovenous COHb gradient successively decreased over time, COHb determined in venous blood underestimated the arterial content.

Original language	English (US)
Pages (from-to)	754-758
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	311
Issue number	3
DOIs	https://doi.org/10.1016/j.bbrc.2003.10.063
State	Published - Nov 21 2003

Keywords

Carbon monoxide poisoning
Carboxyhemoglobin
Critical illness
Heme oxygenase
Hypoxic pulmonary vasoconstriction
Lung
Oxygen saturation
Smoke inhalation

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2003.10.063

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title = "Carboxyhemoglobin formation following smoke inhalation injury in sheep is interrelated with pulmonary shunt fraction",

abstract = "Carboxyhemoglobin (COHb) formation is triggered by the inducible isoform of heme oxygenase (HO-1) catalyzing carbon monoxide (CO) production through breakdown of heme molecules, exposure to CO or both. In the setting of CO poisoning, COHb is regarded as a reliable marker characterizing both severity of injury and efficacy of treatment strategies. This study was designed as a prospective laboratory experiment to elucidate potential interdependencies between COHb generation, oxygenation, and pulmonary shunt fraction (Qs/Qt) in an ovine model of smoke inhalation injury. Chronically instrumented ewes (n=15) were repeatedly subjected to cotton smoke (4×12 breaths) according to an established protocol. This approach resulted in a progressive increase in COHb formation that was interrelated with the degree of Qs/Qt (P<0.001) and inversely correlated with both arterial and mixed venous HbO2 saturation (r=-0.96 and -0.93). Although the arteriovenous COHb gradient successively decreased over time, COHb determined in venous blood underestimated the arterial content.",

keywords = "Carbon monoxide poisoning, Carboxyhemoglobin, Critical illness, Heme oxygenase, Hypoxic pulmonary vasoconstriction, Lung, Oxygen saturation, Smoke inhalation",

author = "Martin Westphal and Naoki Morita and Perenlei Enkhbaatar and Kazunori Murakami and Lillian Traber and Traber, {Daniel Lee}",

note = "Funding Information: This study was supported by the James W. McLaughlin Fund and two intramural Grants: Shriners North America #8450 and #8954.",

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AU - Westphal, Martin

AU - Morita, Naoki

AU - Enkhbaatar, Perenlei

AU - Murakami, Kazunori

AU - Traber, Lillian

AU - Traber, Daniel Lee

N1 - Funding Information: This study was supported by the James W. McLaughlin Fund and two intramural Grants: Shriners North America #8450 and #8954.

PY - 2003/11/21

Y1 - 2003/11/21

N2 - Carboxyhemoglobin (COHb) formation is triggered by the inducible isoform of heme oxygenase (HO-1) catalyzing carbon monoxide (CO) production through breakdown of heme molecules, exposure to CO or both. In the setting of CO poisoning, COHb is regarded as a reliable marker characterizing both severity of injury and efficacy of treatment strategies. This study was designed as a prospective laboratory experiment to elucidate potential interdependencies between COHb generation, oxygenation, and pulmonary shunt fraction (Qs/Qt) in an ovine model of smoke inhalation injury. Chronically instrumented ewes (n=15) were repeatedly subjected to cotton smoke (4×12 breaths) according to an established protocol. This approach resulted in a progressive increase in COHb formation that was interrelated with the degree of Qs/Qt (P<0.001) and inversely correlated with both arterial and mixed venous HbO2 saturation (r=-0.96 and -0.93). Although the arteriovenous COHb gradient successively decreased over time, COHb determined in venous blood underestimated the arterial content.

AB - Carboxyhemoglobin (COHb) formation is triggered by the inducible isoform of heme oxygenase (HO-1) catalyzing carbon monoxide (CO) production through breakdown of heme molecules, exposure to CO or both. In the setting of CO poisoning, COHb is regarded as a reliable marker characterizing both severity of injury and efficacy of treatment strategies. This study was designed as a prospective laboratory experiment to elucidate potential interdependencies between COHb generation, oxygenation, and pulmonary shunt fraction (Qs/Qt) in an ovine model of smoke inhalation injury. Chronically instrumented ewes (n=15) were repeatedly subjected to cotton smoke (4×12 breaths) according to an established protocol. This approach resulted in a progressive increase in COHb formation that was interrelated with the degree of Qs/Qt (P<0.001) and inversely correlated with both arterial and mixed venous HbO2 saturation (r=-0.96 and -0.93). Although the arteriovenous COHb gradient successively decreased over time, COHb determined in venous blood underestimated the arterial content.

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KW - Carboxyhemoglobin

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KW - Heme oxygenase

KW - Hypoxic pulmonary vasoconstriction

KW - Lung

KW - Oxygen saturation

KW - Smoke inhalation

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Carboxyhemoglobin formation following smoke inhalation injury in sheep is interrelated with pulmonary shunt fraction

Abstract

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ASJC Scopus subject areas

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