Cardiovascular collapse and vascular permeability changes in an ovine model of methicillin-resistant staphylococcus aureus sepsis

Collette C. Jonkam, Matthias Lange, Daniel L. Traber, Dirk M. Maybauer, Marc O. Maybauer, Kamna Bansal, Atsumori Hamahata, Yong Zhu, Aimalohi Esechie, Lillian D. Traber, Linda Sousse, Sebastian Rehberg, David Herndon, Perenlei Enkhbaatar

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) infections with severe outcomes such as sepsis and septic shock are progressively increasing in both the community and in hospital settings. We hypothesized that overexpression of reactive nitrogen and oxygen species and vascular endothelial growth factor (VEGF) play a pivotal role in cardiovascular collapse associated with vascular hyperpermeability in MRSA sepsis. Twelve sheep were surgically prepared and randomized into a control (noninjured; n = 6) and a sepsis (injured; n = 6) group. Animals in the sepsis group were subjected to cotton smoke inhalation and instillation of 2.5 × 10 colony-forming units of live MRSA into both lungs. Cardiovascular variables in the control group remained stable, whereas the MRSA sepsis group developed a hypotensive and hyperdynamic circulatory shock state beginning at 6 h associated with significantly increased vascular permeability evidenced by increased prefemoral lymph flow starting at 12 h and permeability index from 12 to 18 h, higher fluid accumulation from 12 to 24 h, and significantly decreased plasma protein concentration and oncotic pressure beginning at 6 h compared with control animals. Myocardial 3-nitrotyrosine (3-NT) protein, poly (adenosine diphosphate-ribose), and VEGF mRNA expressions measured after the 24-h experiment were significantly increased in the injured animals as well. These results evidence that excessive production of reactive radicals and VEGF may play a major role in cardiovascular collapse and vascular hyperpermeability in MRSA sepsis.

Original languageEnglish (US)
Pages (from-to)621-625
Number of pages5
JournalShock
Volume32
Issue number6
DOIs
StatePublished - Dec 2009

Fingerprint

Capillary Permeability
Methicillin-Resistant Staphylococcus aureus
Sheep
Sepsis
Vascular Endothelial Growth Factor A
Blood Vessels
Poly Adenosine Diphosphate Ribose
Reactive Nitrogen Species
Community Hospital
Lymph
Septic Shock
Smoke
Inhalation
Blood Proteins
Shock
Permeability
Reactive Oxygen Species
Stem Cells
Pressure
Lung

Keywords

  • Cardiovascular collapse
  • Microvascular hyperpermeability
  • Reactive nitrogen species
  • Reactive oxygen species
  • VEGF

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine
  • Emergency Medicine

Cite this

Cardiovascular collapse and vascular permeability changes in an ovine model of methicillin-resistant staphylococcus aureus sepsis. / Jonkam, Collette C.; Lange, Matthias; Traber, Daniel L.; Maybauer, Dirk M.; Maybauer, Marc O.; Bansal, Kamna; Hamahata, Atsumori; Zhu, Yong; Esechie, Aimalohi; Traber, Lillian D.; Sousse, Linda; Rehberg, Sebastian; Herndon, David; Enkhbaatar, Perenlei.

In: Shock, Vol. 32, No. 6, 12.2009, p. 621-625.

Research output: Contribution to journalArticle

Jonkam, CC, Lange, M, Traber, DL, Maybauer, DM, Maybauer, MO, Bansal, K, Hamahata, A, Zhu, Y, Esechie, A, Traber, LD, Sousse, L, Rehberg, S, Herndon, D & Enkhbaatar, P 2009, 'Cardiovascular collapse and vascular permeability changes in an ovine model of methicillin-resistant staphylococcus aureus sepsis', Shock, vol. 32, no. 6, pp. 621-625. https://doi.org/10.1097/SHK.0b013e3181a4fd01
Jonkam, Collette C. ; Lange, Matthias ; Traber, Daniel L. ; Maybauer, Dirk M. ; Maybauer, Marc O. ; Bansal, Kamna ; Hamahata, Atsumori ; Zhu, Yong ; Esechie, Aimalohi ; Traber, Lillian D. ; Sousse, Linda ; Rehberg, Sebastian ; Herndon, David ; Enkhbaatar, Perenlei. / Cardiovascular collapse and vascular permeability changes in an ovine model of methicillin-resistant staphylococcus aureus sepsis. In: Shock. 2009 ; Vol. 32, No. 6. pp. 621-625.
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