Cargo-activated ATpase activity of kinesin

Ya Jiang Ming Ya Jiang, Michael Sheetz, J. Gelles, K. Johnson

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

We have measured the ATPase activity of squid optic lobe kinesin bound to polystyrene beads in the presence of microtubules. We find that there is a substantial increase (>10-fold) in the microtubule-activated ATPase activity for bead-bound kinesin over free kinesin. We tentatively attribute such cargo-activated ATPase activity to the presence of a self-inhibited form of kinesin in solution, which becomes activated when bound to a bead in the presence of α-casein. Further experiments are underway to untravel this phenomenon and, in addition, to associate the traveling distance of beads with the observed ATPase rate to determine the average number of ATP consumed per kinesin-bead per micron of travel along microtubule.

Original languageEnglish (US)
JournalBiophysical Journal
Volume68
Issue number4 SUPPL.
StatePublished - Jan 1 1995
Externally publishedYes

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Kinesin
Adenosine Triphosphatases
Microtubules
Decapodiformes
Polystyrenes
Caseins
Adenosine Triphosphate

ASJC Scopus subject areas

  • Biophysics

Cite this

Ming Ya Jiang, Y. J., Sheetz, M., Gelles, J., & Johnson, K. (1995). Cargo-activated ATpase activity of kinesin. Biophysical Journal, 68(4 SUPPL.).

Cargo-activated ATpase activity of kinesin. / Ming Ya Jiang, Ya Jiang; Sheetz, Michael; Gelles, J.; Johnson, K.

In: Biophysical Journal, Vol. 68, No. 4 SUPPL., 01.01.1995.

Research output: Contribution to journalArticle

Ming Ya Jiang, YJ, Sheetz, M, Gelles, J & Johnson, K 1995, 'Cargo-activated ATpase activity of kinesin', Biophysical Journal, vol. 68, no. 4 SUPPL..
Ming Ya Jiang YJ, Sheetz M, Gelles J, Johnson K. Cargo-activated ATpase activity of kinesin. Biophysical Journal. 1995 Jan 1;68(4 SUPPL.).
Ming Ya Jiang, Ya Jiang ; Sheetz, Michael ; Gelles, J. ; Johnson, K. / Cargo-activated ATpase activity of kinesin. In: Biophysical Journal. 1995 ; Vol. 68, No. 4 SUPPL.
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