TY - JOUR
T1 - Carryover effects of larval exposure to different environmental bacteria drive adult trait variation in a mosquito vector
AU - Dickson, Laura B.
AU - Jiolle, Davy
AU - Minard, Guillaume
AU - Moltini-Conclois, Isabelle
AU - Volant, Stevenn
AU - Ghozlane, Amine
AU - Bouchier, Christiane
AU - Ayala, Diego
AU - Paupy, Christophe
AU - Moro, Claire Valiente
AU - Lambrechts, Louis
N1 - Funding Information:
We thank two anonymous reviewers for the helpful comments on an earlier version of the manuscript, N. Rahola and M. F. Ngangue for the assistance and expertise during the fieldwork, A. Fontaine for the help with the statistical analysis, C. Lallemand for the assistance with mosquito rearing, and all members of the Lambrechts laboratory for the insightful discussions. We also thank L. Ma for the technical assistance on the MiSeq and Centre d’Informatique pour la Biologie of Institut Pasteur for providing the computational resources. We are grateful to A. Ponlawat and T. Fansiri for the initial sampling of the mosquito population from Thailand. Funding: This study was supported by the French government’s Investissement d’Avenir program, Laboratoire d’Excellence Integrative Biology of Emerging Infectious Diseases (grant ANR-10-LABX-62-IBEID), the French Agence Nationale de la Recherche (MOSQUIBIOTA project, grant ANR-16-CE35-0004), the City of Paris Emergence(s) program in Biomedical Research, and the French Centre National de la Recherche Scientifique, Ecology of Health (ECOSAN) program (grant AFRICAEDES). The Genomics Facility is a member of the “France Génomique” consortium (grant ANR-10-INBS-09-08). The funders had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. Author contributions: L.B.D., C.V.M., and L.L. conceived the study. L.B.D., D.J., D.A., and C.P. carried out the fieldwork. L.B.D., D.J., and I.M.-C. performed the experiments. C.B. supervised the high-throughput sequencing. L.B.D., G.M., S.V., A.G., and L.L. analyzed the data. L.B.D. and L.L. wrote the manuscript. All authors edited and commented on the manuscript. Competing interests: The authors declare that they have no competing interests. Data and materials availability: All data needed to evaluate the conclusions in the paper are present in the paper and/or the Supplementary Materials and/or have been deposited in the European Nucleotide Archive (accession number PRJEB16334). Materials are available upon request through a material transfer agreement. Additional data related to this paper may be requested from the authors.
Publisher Copyright:
Copyright © 2017 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science.
PY - 2017
Y1 - 2017
N2 - Conditions experienced during larval development of holometabolous insects can affect adult traits, but whether differences in the bacterial communities of larval development sites contribute to variation in the ability of insect vectors to transmit human pathogens is unknown. We addressed this question in the mosquito Aedes aegypti, a major arbovirus vector breeding in both sylvatic and domestic habitats in Sub-Saharan Africa. Targeted metagenomics revealed differing bacterial communities in the water of natural breeding sites in Gabon. Experimental exposure to different native bacterial isolates during larval development resulted in significant differences in pupation rate and adult body size but not life span. Larval exposure to an Enterobacteriaceae isolate resulted in decreased antibacterial activity in adult hemolymph and reduced dengue virus dissemination titer. Together, these data provide the proof of concept that larval exposure to different bacteria can drive variation in adult traits underlying vectorial capacity. Our study establishes a functional link between larval ecology, environmental microbes, and adult phenotypic variation in a holometabolous insect vector.
AB - Conditions experienced during larval development of holometabolous insects can affect adult traits, but whether differences in the bacterial communities of larval development sites contribute to variation in the ability of insect vectors to transmit human pathogens is unknown. We addressed this question in the mosquito Aedes aegypti, a major arbovirus vector breeding in both sylvatic and domestic habitats in Sub-Saharan Africa. Targeted metagenomics revealed differing bacterial communities in the water of natural breeding sites in Gabon. Experimental exposure to different native bacterial isolates during larval development resulted in significant differences in pupation rate and adult body size but not life span. Larval exposure to an Enterobacteriaceae isolate resulted in decreased antibacterial activity in adult hemolymph and reduced dengue virus dissemination titer. Together, these data provide the proof of concept that larval exposure to different bacteria can drive variation in adult traits underlying vectorial capacity. Our study establishes a functional link between larval ecology, environmental microbes, and adult phenotypic variation in a holometabolous insect vector.
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U2 - 10.1126/sciadv.1700585
DO - 10.1126/sciadv.1700585
M3 - Article
C2 - 28835919
AN - SCOPUS:85038033031
SN - 2375-2548
VL - 3
JO - Science advances
JF - Science advances
IS - 8
M1 - 1700585
ER -