Cat phrenic nucleus architecture as revealed by horseradish peroxidase mapping

Ch L. Webber, R. D. Wurste, Jin Chung

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Cat phrenic motoneurons, labeled by intradiaphragmatic injection of horseradish peroxidase, formed a tight cluster in the most ventral portion of the ventral horn in lamina IX of the lower cervical cord. Cell counts were symmetrically distributed for 17 to 21 mm along the longitudinal axis of the cord with a unimodal peak at the junction of segments C5 and C6. The phrenic nucleus was bilaterally organized on either side of the cord with anatomical symmetry and in no case was there evidence for the crossing of phrenic axons in the cord. Assessment of cellular geometry and intercellular relationships demonstrated that phrenic cell diameters approximated a normal distribution with a single peak at 26 μm while longitudinal cell lengths averaged 76 μm. Cells of different size were mixed randomly at all levels of the nucleus. The minimum distance between cells was about 10 μm and the maximum cell packing density approached 2 cells per 106 μm3. The results confirm the location of the cat phrenic nucleus, extend the knowledge of phrenic motoneuronal geometry, and provide an anatomical basis for the understanding of recruitment and synchronization phenomena within the phrenic nucleus.

Original languageEnglish (US)
Pages (from-to)395-406
Number of pages12
JournalExperimental Brain Research
Volume35
Issue number3
DOIs
StatePublished - May 1979
Externally publishedYes

Fingerprint

Horseradish Peroxidase
Diaphragm
Cats
Cell Count
Normal Distribution
Motor Neurons
Horns
Cell Size
Axons
Injections
Cervical Cord

Keywords

  • Cat
  • Horseradish peroxidase
  • Neuron geometry
  • Phrenic motoneurons
  • Spinal cord

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Cat phrenic nucleus architecture as revealed by horseradish peroxidase mapping. / Webber, Ch L.; Wurste, R. D.; Chung, Jin.

In: Experimental Brain Research, Vol. 35, No. 3, 05.1979, p. 395-406.

Research output: Contribution to journalArticle

@article{e0ef10fe094b431b8b4d2eb99d07e902,
title = "Cat phrenic nucleus architecture as revealed by horseradish peroxidase mapping",
abstract = "Cat phrenic motoneurons, labeled by intradiaphragmatic injection of horseradish peroxidase, formed a tight cluster in the most ventral portion of the ventral horn in lamina IX of the lower cervical cord. Cell counts were symmetrically distributed for 17 to 21 mm along the longitudinal axis of the cord with a unimodal peak at the junction of segments C5 and C6. The phrenic nucleus was bilaterally organized on either side of the cord with anatomical symmetry and in no case was there evidence for the crossing of phrenic axons in the cord. Assessment of cellular geometry and intercellular relationships demonstrated that phrenic cell diameters approximated a normal distribution with a single peak at 26 μm while longitudinal cell lengths averaged 76 μm. Cells of different size were mixed randomly at all levels of the nucleus. The minimum distance between cells was about 10 μm and the maximum cell packing density approached 2 cells per 106 μm3. The results confirm the location of the cat phrenic nucleus, extend the knowledge of phrenic motoneuronal geometry, and provide an anatomical basis for the understanding of recruitment and synchronization phenomena within the phrenic nucleus.",
keywords = "Cat, Horseradish peroxidase, Neuron geometry, Phrenic motoneurons, Spinal cord",
author = "Webber, {Ch L.} and Wurste, {R. D.} and Jin Chung",
year = "1979",
month = "5",
doi = "10.1007/BF00236759",
language = "English (US)",
volume = "35",
pages = "395--406",
journal = "Experimental Brain Research",
issn = "0014-4819",
publisher = "Springer Verlag",
number = "3",

}

TY - JOUR

T1 - Cat phrenic nucleus architecture as revealed by horseradish peroxidase mapping

AU - Webber, Ch L.

AU - Wurste, R. D.

AU - Chung, Jin

PY - 1979/5

Y1 - 1979/5

N2 - Cat phrenic motoneurons, labeled by intradiaphragmatic injection of horseradish peroxidase, formed a tight cluster in the most ventral portion of the ventral horn in lamina IX of the lower cervical cord. Cell counts were symmetrically distributed for 17 to 21 mm along the longitudinal axis of the cord with a unimodal peak at the junction of segments C5 and C6. The phrenic nucleus was bilaterally organized on either side of the cord with anatomical symmetry and in no case was there evidence for the crossing of phrenic axons in the cord. Assessment of cellular geometry and intercellular relationships demonstrated that phrenic cell diameters approximated a normal distribution with a single peak at 26 μm while longitudinal cell lengths averaged 76 μm. Cells of different size were mixed randomly at all levels of the nucleus. The minimum distance between cells was about 10 μm and the maximum cell packing density approached 2 cells per 106 μm3. The results confirm the location of the cat phrenic nucleus, extend the knowledge of phrenic motoneuronal geometry, and provide an anatomical basis for the understanding of recruitment and synchronization phenomena within the phrenic nucleus.

AB - Cat phrenic motoneurons, labeled by intradiaphragmatic injection of horseradish peroxidase, formed a tight cluster in the most ventral portion of the ventral horn in lamina IX of the lower cervical cord. Cell counts were symmetrically distributed for 17 to 21 mm along the longitudinal axis of the cord with a unimodal peak at the junction of segments C5 and C6. The phrenic nucleus was bilaterally organized on either side of the cord with anatomical symmetry and in no case was there evidence for the crossing of phrenic axons in the cord. Assessment of cellular geometry and intercellular relationships demonstrated that phrenic cell diameters approximated a normal distribution with a single peak at 26 μm while longitudinal cell lengths averaged 76 μm. Cells of different size were mixed randomly at all levels of the nucleus. The minimum distance between cells was about 10 μm and the maximum cell packing density approached 2 cells per 106 μm3. The results confirm the location of the cat phrenic nucleus, extend the knowledge of phrenic motoneuronal geometry, and provide an anatomical basis for the understanding of recruitment and synchronization phenomena within the phrenic nucleus.

KW - Cat

KW - Horseradish peroxidase

KW - Neuron geometry

KW - Phrenic motoneurons

KW - Spinal cord

UR - http://www.scopus.com/inward/record.url?scp=0018418415&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0018418415&partnerID=8YFLogxK

U2 - 10.1007/BF00236759

DO - 10.1007/BF00236759

M3 - Article

C2 - 456448

AN - SCOPUS:0018418415

VL - 35

SP - 395

EP - 406

JO - Experimental Brain Research

JF - Experimental Brain Research

SN - 0014-4819

IS - 3

ER -