Catabolism of adenine nucleotides in adenosine deaminase deficient erythrocytes

Gordon C. Mills, Randall M. Goldblum, Frank C. Schmalstieg

Research output: Contribution to journalArticle

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Abstract

In vitro incubation studies using fluoride and iodoacetate as glycolytic inhibitors have been carried out on red cells of the two subjects with adenosine deaminase deficiency. For comparison, similar studies have also been carried out on red cells from a normal subject and from a child with severe combined immunodeficiency with normal adenosine deaminase activity. The adenosine formed in the adenosine deaminase deficient red cells is a measure of adenosine 5′-phosphate breakdown initiated by 5′-nucleotidase, whereas inosine 5′-phosphate, inosine and hypoxanthine formation is a measure of adenosine 5′-phosphate breakdown initiated by adenylate deaminase. With fluoride as inhibitor, nearly all of the adenosine 5′-phosphate breakdown proceeded by way of adenylate deaminase, while with iodoacetate as inhibitor, 20-30% of the adenosine 5′-phosphate breakdown was initiated by 5′-nucleotidase acting on adenosine 5′-phosphate. In addition, significant amounts of adenine were produced in adenosine deaminase deficient red cells in the presence of the glycolytic inhibitors. Possible explanations for the findings noted in this study are discussed and related to recent studies on the properties of the pertinent purine nucleotide catabolic enzymes.

Original languageEnglish (US)
Pages (from-to)1811-1820
Number of pages10
JournalLife Sciences
Volume29
Issue number17
DOIs
StatePublished - Oct 26 1981

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Adenosine Deaminase
Adenine Nucleotides
Adenosine Monophosphate
Erythrocytes
Cells
AMP Deaminase
Iodoacetates
5'-Nucleotidase
Fluorides
Inosine Nucleotides
Purine Nucleotides
Severe Combined Immunodeficiency
Inosine
Hypoxanthine
Adenine
Adenosine
Enzymes

ASJC Scopus subject areas

  • Pharmacology

Cite this

Catabolism of adenine nucleotides in adenosine deaminase deficient erythrocytes. / Mills, Gordon C.; Goldblum, Randall M.; Schmalstieg, Frank C.

In: Life Sciences, Vol. 29, No. 17, 26.10.1981, p. 1811-1820.

Research output: Contribution to journalArticle

Mills, Gordon C. ; Goldblum, Randall M. ; Schmalstieg, Frank C. / Catabolism of adenine nucleotides in adenosine deaminase deficient erythrocytes. In: Life Sciences. 1981 ; Vol. 29, No. 17. pp. 1811-1820.
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