Cation effects on cell shape.

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We have found that human erythrocyte ghosts in 10 mM HEPES (pH 7.0) at 0 degrees C would crenate when 20-50 mM of Na+ or K+, 0.2-0.5 mM OF Ca++, Ba++, Sr++, or Mg++, or 10 muM of La+++ was added. The shape change after cation addition was faster than fixation by 1% glutaraldehyde at 4 degrees C and was readily reversible upon dilution of the cation. After incubation of ghosts in 10 mM HEPES (pH 7.0) at 37 degrees for 10-20 min there was a significant inhibition of subsequent crenation by cations. In a process that is believed to occur by a similar mechanism, whole red blood cells were observed to cup (invaginate) when 20 mM of a divalent or 0.1 mM of a trivalent cation was added. After neuraminidase treatment to remove the sialic acid charge groups, these same shape changes were observed in ghosts and whole cells. Another type of cation-induced crenation was found to follow upon the addition to whole cells of A23187 and Ca++ or Ba++ but not Mg++. This process is much slower than crenation in the ghost and is believed to be caused by a different mechanism.

Original languageEnglish (US)
Pages (from-to)559-567
Number of pages9
JournalProgress in clinical and biological research
Volume17
StatePublished - Dec 1 1977
Externally publishedYes

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Cell Shape
Cations
HEPES
Calcimycin
Erythrocyte Membrane
Neuraminidase
Glutaral
N-Acetylneuraminic Acid
Erythrocytes

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Cation effects on cell shape. / Sheetz, Michael.

In: Progress in clinical and biological research, Vol. 17, 01.12.1977, p. 559-567.

Research output: Contribution to journalArticle

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