Cationic lipid-mediated NGF gene transfection increases neurofilament phosphorylation

K. Yang, J. J. Xue, Y. H. Qiu, J. R. Perez-Polo, J. Whitson, F. Faustinella, A. Kampfl, X. Zhao, Y. Iwamoto, X. S. Mu, G. Clifton, R. L. Hayes

    Research output: Contribution to journalArticlepeer-review

    8 Scopus citations

    Abstract

    We examined the effect of cationic lipid-mediated gene transfection of nerve growth factor (NGF) in primary septo-hippocampal cell cultures. Rat NGF cDNA was subcloned into a pUC19-based plasmid containing a CMV promoter. Two days after NGF gene transfection in primary cell cultures, ELISA confirmed increases in NGF protein secretion from transfected cells. To study the biological effect of cationic lipid-mediated NGF gene transfection, we analyzed the amount of neurofilament protein from NGF-transfected cell cultures. Western blot and immunohistochemical analyses detected significant increases in the phosphorylated form of neurofilament proteins in the cultures after cationic lipid-mediated NGF cDNA transfection. Cationic lipid-mediated NGF cDNA transfection aid not cause significant changes in the total amount of neurofilament protein. Our studies suggest that cationic lipid-mediated NGF gene transfection can increase neurofilament phosphorylation but not total neurofilament protein.

    Original languageEnglish (US)
    Pages (from-to)13-20
    Number of pages8
    JournalMolecular Brain Research
    Volume43
    Issue number1-2
    DOIs
    StatePublished - Dec 31 1996

    Keywords

    • Cationic lipid
    • Cell culture
    • Gene transfer
    • Nerve growth factor
    • Neurofilament
    • Septo-hippocampal system

    ASJC Scopus subject areas

    • Molecular Biology
    • Cellular and Molecular Neuroscience

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