Cationic lipid-mediated NGF gene transfection increases neurofilament phosphorylation

K. Yang, J. J. Xue, Y. H. Qiu, J. R. Perez-Polo, J. Whitson, F. Faustinella, A. Kampfl, X. Zhao, Y. Iwamoto, X. S. Mu, G. Clifton, R. L. Hayes

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

We examined the effect of cationic lipid-mediated gene transfection of nerve growth factor (NGF) in primary septo-hippocampal cell cultures. Rat NGF cDNA was subcloned into a pUC19-based plasmid containing a CMV promoter. Two days after NGF gene transfection in primary cell cultures, ELISA confirmed increases in NGF protein secretion from transfected cells. To study the biological effect of cationic lipid-mediated NGF gene transfection, we analyzed the amount of neurofilament protein from NGF-transfected cell cultures. Western blot and immunohistochemical analyses detected significant increases in the phosphorylated form of neurofilament proteins in the cultures after cationic lipid-mediated NGF cDNA transfection. Cationic lipid-mediated NGF cDNA transfection aid not cause significant changes in the total amount of neurofilament protein. Our studies suggest that cationic lipid-mediated NGF gene transfection can increase neurofilament phosphorylation but not total neurofilament protein.

Original languageEnglish (US)
Pages (from-to)13-20
Number of pages8
JournalMolecular Brain Research
Volume43
Issue number1-2
DOIs
StatePublished - Dec 31 1996
Externally publishedYes

Keywords

  • Cationic lipid
  • Cell culture
  • Gene transfer
  • Nerve growth factor
  • Neurofilament
  • Septo-hippocampal system

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience

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