Cationic lipid-mediated NGF gene transfection increases neurofilament phosphorylation

K. Yang, J. J. Xue, Y. H. Qiu, J. R. Perez-Polo, J. Whitson, F. Faustinella, A. Kampfl, X. Zhao, Y. Iwamoto, X. S. Mu, G. Clifton, R. L. Hayes

    Research output: Contribution to journalArticle

    8 Citations (Scopus)

    Abstract

    We examined the effect of cationic lipid-mediated gene transfection of nerve growth factor (NGF) in primary septo-hippocampal cell cultures. Rat NGF cDNA was subcloned into a pUC19-based plasmid containing a CMV promoter. Two days after NGF gene transfection in primary cell cultures, ELISA confirmed increases in NGF protein secretion from transfected cells. To study the biological effect of cationic lipid-mediated NGF gene transfection, we analyzed the amount of neurofilament protein from NGF-transfected cell cultures. Western blot and immunohistochemical analyses detected significant increases in the phosphorylated form of neurofilament proteins in the cultures after cationic lipid-mediated NGF cDNA transfection. Cationic lipid-mediated NGF cDNA transfection aid not cause significant changes in the total amount of neurofilament protein. Our studies suggest that cationic lipid-mediated NGF gene transfection can increase neurofilament phosphorylation but not total neurofilament protein.

    Original languageEnglish (US)
    Pages (from-to)13-20
    Number of pages8
    JournalMolecular Brain Research
    Volume43
    Issue number1-2
    DOIs
    StatePublished - Dec 31 1996

    Fingerprint

    Intermediate Filaments
    Nerve Growth Factor
    Transfection
    Phosphorylation
    Lipids
    Neurofilament Proteins
    Genes
    Complementary DNA
    Cell Culture Techniques
    Primary Cell Culture
    Plasmids
    Western Blotting
    Enzyme-Linked Immunosorbent Assay

    Keywords

    • Cationic lipid
    • Cell culture
    • Gene transfer
    • Nerve growth factor
    • Neurofilament
    • Septo-hippocampal system

    ASJC Scopus subject areas

    • Molecular Biology
    • Cellular and Molecular Neuroscience

    Cite this

    Yang, K., Xue, J. J., Qiu, Y. H., Perez-Polo, J. R., Whitson, J., Faustinella, F., ... Hayes, R. L. (1996). Cationic lipid-mediated NGF gene transfection increases neurofilament phosphorylation. Molecular Brain Research, 43(1-2), 13-20. https://doi.org/10.1016/S0169-328X(96)00142-8

    Cationic lipid-mediated NGF gene transfection increases neurofilament phosphorylation. / Yang, K.; Xue, J. J.; Qiu, Y. H.; Perez-Polo, J. R.; Whitson, J.; Faustinella, F.; Kampfl, A.; Zhao, X.; Iwamoto, Y.; Mu, X. S.; Clifton, G.; Hayes, R. L.

    In: Molecular Brain Research, Vol. 43, No. 1-2, 31.12.1996, p. 13-20.

    Research output: Contribution to journalArticle

    Yang, K, Xue, JJ, Qiu, YH, Perez-Polo, JR, Whitson, J, Faustinella, F, Kampfl, A, Zhao, X, Iwamoto, Y, Mu, XS, Clifton, G & Hayes, RL 1996, 'Cationic lipid-mediated NGF gene transfection increases neurofilament phosphorylation', Molecular Brain Research, vol. 43, no. 1-2, pp. 13-20. https://doi.org/10.1016/S0169-328X(96)00142-8
    Yang, K. ; Xue, J. J. ; Qiu, Y. H. ; Perez-Polo, J. R. ; Whitson, J. ; Faustinella, F. ; Kampfl, A. ; Zhao, X. ; Iwamoto, Y. ; Mu, X. S. ; Clifton, G. ; Hayes, R. L. / Cationic lipid-mediated NGF gene transfection increases neurofilament phosphorylation. In: Molecular Brain Research. 1996 ; Vol. 43, No. 1-2. pp. 13-20.
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