Caveolin-1 and lipid microdomains regulate Gs trafficking and attenuate Gs/adenylyl cyclase signaling

John Allen, Jiang Z. Yu, Rahul H. Dave, Anushree Bhatnagar, Bryan L. Roth, Mark M. Rasenick

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Lipid rafts and caveolae are specialized membrane microdomains implicated in regulating G protein-coupled receptor signaling cascades. Previous studies have suggested that rafts/ caveolae may regulate β-adrenergic receptor/Gαs signaling, but underlying molecular mechanisms are largely undefined. Using a simplified model system in C6 glioma cells, this study disrupts rafts/caveolae using both pharmacological and genetic approaches to test whether caveolin-1 and lipid microdomains regulate Gs trafficking and signaling. Lipid rafts/caveolae were disrupted in C6 cells by either short-term cholesterol chelation using methyl-β-cyclodextrin or by stable knockdown of caveolin-1 and -2 by RNA interference. In imaging studies examining Gαs-GFP during signaling, stimulation with the βAR agonist isoproterenol resulted in internalization of Gαs-GFP; however, this trafficking was blocked by methyl-β-cyclodextrin or by caveolin knockdown. Caveolin knockdown significantly decreased Gαs localization in detergent insoluble lipid raft/caveolae membrane fractions, suggesting that caveolin localizes a portion of Gαs to these membrane microdomains. Methyl-β-cyclodextrin or caveolin knockdown significantly increased isoproterenol or thyrotropin- stimulated cAMP accumulation. Furthermore, forskolin- and aluminum tetrafluoride-stimulated adenylyl cyclase activity was significantly increased by caveolin knockdown in cells or in brain membranes obtained from caveolin-1 knockout mice, indicating that caveolin attenuates signaling at the level of Gαs/ adenylyl cyclase and distal to GPCRs. Taken together, these results demonstrate that caveolin-1 and lipid microdomains exert a major effect on Gαs trafficking and signaling. It is suggested that lipid rafts/caveolae are sites that remove Gαs from membrane signaling cascades and caveolins might dampen globally Gαs/ adenylyl cyclase/cAMP signaling.

Original languageEnglish (US)
Pages (from-to)1082-1093
Number of pages12
JournalMolecular Pharmacology
Volume76
Issue number5
DOIs
StatePublished - Nov 2009
Externally publishedYes

Fingerprint

Caveolins
Caveolin 1
Caveolae
Adenylyl Cyclases
Lipids
Cyclodextrins
Membrane Microdomains
Isoproterenol
Membranes
Caveolin 2
Colforsin
Thyrotropin
G-Protein-Coupled Receptors
RNA Interference
Knockout Mice
Glioma
Detergents
Adrenergic Receptors
Cholesterol
Pharmacology

ASJC Scopus subject areas

  • Pharmacology
  • Molecular Medicine

Cite this

Caveolin-1 and lipid microdomains regulate Gs trafficking and attenuate Gs/adenylyl cyclase signaling. / Allen, John; Yu, Jiang Z.; Dave, Rahul H.; Bhatnagar, Anushree; Roth, Bryan L.; Rasenick, Mark M.

In: Molecular Pharmacology, Vol. 76, No. 5, 11.2009, p. 1082-1093.

Research output: Contribution to journalArticle

Allen, John ; Yu, Jiang Z. ; Dave, Rahul H. ; Bhatnagar, Anushree ; Roth, Bryan L. ; Rasenick, Mark M. / Caveolin-1 and lipid microdomains regulate Gs trafficking and attenuate Gs/adenylyl cyclase signaling. In: Molecular Pharmacology. 2009 ; Vol. 76, No. 5. pp. 1082-1093.
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