Caveolin-1 induces lamellipodia formation via an Akt-dependent pathway

Pithi Chanvorachote, Preedakorn Chunhacha, Varisa Pongrakhananon

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Background: The enhancement of migration is critical for facilitating cancer cell metastasis.Method: Lung cancer H23 cells were transfected with either a caveolin-1 (Cav-1) overexpression or shCav-1 plasmid and further subjected to cell migration assays and lamellipodia characterization. The regulation of Cav-1 via an ATP-dependent tyrosine kinase (Akt) pathway was further examined by Akt knockdown in Cav-1 overexpressing cells and migratory behavior investigations.Results: Here, we demonstrate for the first time that overexpression of Cav-1 in human lung cancer H23 cells significantly increased the formation of lamellipodia, whereas the suppression of Cav-1 using shRNA transfection had the opposite effect. Consistent with an increase in lamellipodia, Cav-1 overexpressing cells exhibited increased migratory activity in comparison to their parental, control-transfected, H23 cells. The induction of lamellipodia was demonstrated to occur via the Akt pathway because the addition of the Akt inhibitor LY294002 inhibited lamellipodia in both Cav-1-overexpressing and H23 cells. Additionally, transient transfection with Akt-siRNA significantly inhibited the formation of lamellipodia and the migration of Cav-1-overexpressing H23 cells. In addition, Cav-1 levels and the migratory action of other lung cancer cells, namely, H460 and A549, were assessed, and the migration of these cells was found to be correlated with the basal Cav-1 level.Conclusion: These data showed that Cav-1 enhances cancer cell migration through Akt-mediated lamellipodia formation. Our results provide novel insights regarding the molecular mechanism controlling cancer cell migration, leading to a better understanding of cancer cell biology.

Original languageEnglish (US)
Article number52
JournalCancer Cell International
Volume14
Issue number1
DOIs
StatePublished - Jun 14 2014
Externally publishedYes

Fingerprint

Caveolin 1
Pseudopodia
Cell Movement
Lung Neoplasms
Small Interfering RNA
Transfection
Neoplasms
Cell Migration Assays
2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one
Protein-Tyrosine Kinases
Cell Biology
Plasmids
Adenosine Triphosphate

Keywords

  • Cancer migration
  • Caveolin-1
  • Lamellipodia
  • Lung cancer

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Genetics

Cite this

Caveolin-1 induces lamellipodia formation via an Akt-dependent pathway. / Chanvorachote, Pithi; Chunhacha, Preedakorn; Pongrakhananon, Varisa.

In: Cancer Cell International, Vol. 14, No. 1, 52, 14.06.2014.

Research output: Contribution to journalArticle

Chanvorachote, Pithi ; Chunhacha, Preedakorn ; Pongrakhananon, Varisa. / Caveolin-1 induces lamellipodia formation via an Akt-dependent pathway. In: Cancer Cell International. 2014 ; Vol. 14, No. 1.
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