Caveolin-1 Regulates Endothelial Adhesion of Lung Cancer Cells via Reactive Oxygen Species-Dependent Mechanism

Pithi Chanvorachote, Preedakorn Chunhacha

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The knowledge regarding the role of caveolin-1 (Cav-1) protein on endothelium adhesion of cancer cells is unclear. The present study revealed that Cav-1 plays a negative regulatory role on cancer-endothelium interaction. Endogenous Cav-1 was shown to down-regulate during cell detachment and the level of such a protein was conversely associated with tumor-endothelial adhesion. Furthermore, the ectopic overexpression of Cav-1 attenuated the ability of the cancer cells to adhere to endothelium while shRNA-mediated Cav-1 knock-down exhibited the opposite effect. We found that cell detachment increased cellular hydrogen peroxide and hydroxyl radical generation and such reactive oxygen species (ROS) were responsible for the increasing interaction between cancer cells and endothelial cells through vascular endothelial cell adhesion molecule-1 (VCAM-1). Importantly, Cav-1 was shown to suppress hydrogen peroxide and hydroxyl radical formation by sustaining the level of activated Akt which was critical for the role of Cav-1 in attenuating the cell adhesion. Together, the present study revealed the novel role of Cav-1 and underlying mechanism on tumor adhesion which explain and highlight an important role of Cav-1 on lung cancer cell metastasis.

Original languageEnglish (US)
Article numbere57466
JournalPLoS One
Volume8
Issue number2
DOIs
StatePublished - Feb 27 2013
Externally publishedYes

Fingerprint

Caveolin 1
lung neoplasms
adhesion
reactive oxygen species
Reactive Oxygen Species
Lung Neoplasms
Adhesion
endothelium
Cells
hydroxyl radicals
cell adhesion
endothelial cells
neoplasms
hydrogen peroxide
Endothelium
Neoplasms
Endothelial cells
blood vessels
metastasis
Cell Adhesion

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Caveolin-1 Regulates Endothelial Adhesion of Lung Cancer Cells via Reactive Oxygen Species-Dependent Mechanism. / Chanvorachote, Pithi; Chunhacha, Preedakorn.

In: PLoS One, Vol. 8, No. 2, e57466, 27.02.2013.

Research output: Contribution to journalArticle

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