CCL2/monocyte chemoattractant protein-1 mediates enhanced transmigration of human immunodeficiency virus (HIV)-infected leukocytes across the blood-brain barrier

A potential mechanism of HIV-CNS invasion and NeuroAIDS

Eliseo Eugenin, Kristin Osiecki, Lillie Lopez, Harris Goldstein, Tina M. Calderon, Joan W. Berman

Research output: Contribution to journalArticle

254 Citations (Scopus)

Abstract

Encephalitis and dementia associated with acquired immunodeficiency syndrome (AIDS) are characterized by leukocyte infiltration into the CNS, microglia activation, aberrant chemokine expression, blood-brain barrier (BBB) disruption, and eventual loss of neurons. Little is known about whether human immunodeficiency virus 1 (HIV-1) infection of leukocytes affects their ability to transmigrate in response to chemokines and to alter BBB integrity. We now demonstrate that HIV infection of human leukocytes results in their increased transmigration across our tissue culture model of the human BBB in response to the chemokine CCL2, as well as in disruption of the BBB, as evidenced by enhanced permeability, reduction of tight junction proteins, and expression of matrix metalloproteinases (MMP)-2 and MMP-9. HIV-infected cells added to our model did not transmigrate in the absence of CCL2, nor did this condition alterBBBintegrity. The chemokines CXCL10/interferon-gamma-inducible protein of 10 kDa, CCL3/macrophage inflammatory protein-1α, or CCL5/RANTES (regulated on activation normal T-cell expressed and secreted) did not enhance HIV-infected leukocyte transmigration or BBB permeability. The increased capacity of HIV-infected leukocytes to transmigrate in response to CCL2 correlated with their increased expression of CCR2, the chemokine receptor for CCL2. These data suggest that CCL2, but not other chemokines, plays a key role in infiltration of HIV-infected leukocytes into the CNS and the subsequent pathology characteristic of NeuroAIDS.

Original languageEnglish (US)
Pages (from-to)1098-1106
Number of pages9
JournalJournal of Neuroscience
Volume26
Issue number4
DOIs
StatePublished - Jan 25 2006
Externally publishedYes

Fingerprint

Chemokine CCL2
Blood-Brain Barrier
Leukocytes
HIV
Chemokines
Chemokine CXCL10
Virus Diseases
Permeability
CCR2 Receptors
Tight Junction Proteins
Macrophage Inflammatory Proteins
Chemokine CCL5
Aptitude
Chemokine Receptors
Matrix Metalloproteinase 2
Matrix Metalloproteinase 9
Microglia
Encephalitis
Dementia
HIV-1

Keywords

  • Blood-brain barrier
  • Cell trafficking
  • Chemokines
  • HIV
  • Macrophage-monocytes
  • Monocytes
  • NeuroAIDS

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

CCL2/monocyte chemoattractant protein-1 mediates enhanced transmigration of human immunodeficiency virus (HIV)-infected leukocytes across the blood-brain barrier : A potential mechanism of HIV-CNS invasion and NeuroAIDS. / Eugenin, Eliseo; Osiecki, Kristin; Lopez, Lillie; Goldstein, Harris; Calderon, Tina M.; Berman, Joan W.

In: Journal of Neuroscience, Vol. 26, No. 4, 25.01.2006, p. 1098-1106.

Research output: Contribution to journalArticle

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abstract = "Encephalitis and dementia associated with acquired immunodeficiency syndrome (AIDS) are characterized by leukocyte infiltration into the CNS, microglia activation, aberrant chemokine expression, blood-brain barrier (BBB) disruption, and eventual loss of neurons. Little is known about whether human immunodeficiency virus 1 (HIV-1) infection of leukocytes affects their ability to transmigrate in response to chemokines and to alter BBB integrity. We now demonstrate that HIV infection of human leukocytes results in their increased transmigration across our tissue culture model of the human BBB in response to the chemokine CCL2, as well as in disruption of the BBB, as evidenced by enhanced permeability, reduction of tight junction proteins, and expression of matrix metalloproteinases (MMP)-2 and MMP-9. HIV-infected cells added to our model did not transmigrate in the absence of CCL2, nor did this condition alterBBBintegrity. The chemokines CXCL10/interferon-gamma-inducible protein of 10 kDa, CCL3/macrophage inflammatory protein-1α, or CCL5/RANTES (regulated on activation normal T-cell expressed and secreted) did not enhance HIV-infected leukocyte transmigration or BBB permeability. The increased capacity of HIV-infected leukocytes to transmigrate in response to CCL2 correlated with their increased expression of CCR2, the chemokine receptor for CCL2. These data suggest that CCL2, but not other chemokines, plays a key role in infiltration of HIV-infected leukocytes into the CNS and the subsequent pathology characteristic of NeuroAIDS.",
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