CD11b blockade prevents lung injury despite neutrophil priming after gut ischemia/reperfusion

K. Koike, E. E. Moore, F. A. Moore, R. J. Franciose, B. Fontes, F. J. Kim, William Mileski, S. R. Shackford, R. V. Maier, H. O. Rennekampff

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Gut ischemia/reperfusion (I/R) provokes lung injury via a mechanism that involves neutrophils [polymorphonuclear neutrophils (PMNs)]. CD11b/CD18 (αmB2) is the integrin receptor on PMNs critical for adhesion-dependent oxidative burst. The purpose of this study was to investigate the mechanistic role of CD11b in the process of gut I/R-induced lung injury. Sprague-Dawley rats underwent 45 minutes of superior mesenteric artery (SMA) occlusion with and without CD11b monoclonal antibody treatment (IB6) (1 mg/kg, IV), before SMA clamping. At 2-hour reperfusion, PMN presence in tissue was quantitated by myeloperoxidase activity and circulating PMN priming determined by the difference in superoxide production with and without N-formyl-methionyl- leucyl-phenylalanine, whereas lung leak was assessed by 125I-albumin lung/blood ratio. In sum, CD11b blockade prevented gut I/R-induced lung leak, but did not attenuate gut I/R-induced PMN priming or tissue PMN accumulation. In conclusion, gut I/R promotes PMN priming and PMN adhesion in both local and distant beds via receptors other than CD11b, but this B2 integrin receptor is critical for PMN-mediated endothelial injury.

Original languageEnglish (US)
Pages (from-to)23-26
Number of pages4
JournalJournal of Trauma - Injury, Infection and Critical Care
Volume39
Issue number1
DOIs
StatePublished - 1995
Externally publishedYes

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Lung Injury
Reperfusion
Neutrophils
Ischemia
Superior Mesenteric Artery
Integrins
Lung
N-Formylmethionine Leucyl-Phenylalanine
Respiratory Burst
Constriction
Superoxides
Peroxidase
Sprague Dawley Rats
Albumins
Monoclonal Antibodies
Wounds and Injuries

ASJC Scopus subject areas

  • Surgery

Cite this

CD11b blockade prevents lung injury despite neutrophil priming after gut ischemia/reperfusion. / Koike, K.; Moore, E. E.; Moore, F. A.; Franciose, R. J.; Fontes, B.; Kim, F. J.; Mileski, William; Shackford, S. R.; Maier, R. V.; Rennekampff, H. O.

In: Journal of Trauma - Injury, Infection and Critical Care, Vol. 39, No. 1, 1995, p. 23-26.

Research output: Contribution to journalArticle

Koike, K, Moore, EE, Moore, FA, Franciose, RJ, Fontes, B, Kim, FJ, Mileski, W, Shackford, SR, Maier, RV & Rennekampff, HO 1995, 'CD11b blockade prevents lung injury despite neutrophil priming after gut ischemia/reperfusion', Journal of Trauma - Injury, Infection and Critical Care, vol. 39, no. 1, pp. 23-26. https://doi.org/10.1097/00005373-199507000-00003
Koike, K. ; Moore, E. E. ; Moore, F. A. ; Franciose, R. J. ; Fontes, B. ; Kim, F. J. ; Mileski, William ; Shackford, S. R. ; Maier, R. V. ; Rennekampff, H. O. / CD11b blockade prevents lung injury despite neutrophil priming after gut ischemia/reperfusion. In: Journal of Trauma - Injury, Infection and Critical Care. 1995 ; Vol. 39, No. 1. pp. 23-26.
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