CD177+ neutrophils suppress epithelial cell tumourigenesis in colitis-associated cancer and predict good prognosis in colorectal cancer

Guangxi Zhou, Kangsheng Peng, Yang Song, Wenjing Yang, Weigang Shu, Tianming Yu, Lin Yu, Moubin Lin, Qing Wei, Chunqiu Chen, Lu Yin, Yingzi Cong, Zhanju Liu

    Research output: Contribution to journalArticle

    6 Citations (Scopus)

    Abstract

    Neutrophils are found to be infiltrated in tumour tissues of patients with colitis-associated cancer (CAC) and colorectal cancer (CRC), and CD177 is mainly expressed in neutrophils. In our study, expression of CD177 in tumour tissues from patients with CAC or CRC was analysed byquantitative real-time polymerase chain reaction, flow cytometry and immunohistochemistry. We recruited 378 patients with CRC, determined CD177 expression in tumours and examined its correlation with clinicopathological features. Moreover, CAC model was induced in wild-type and CD177-/- mice by azoxymethane/dextran sodium sulphate. CD177+ neutrophils were significantly increased in colon tumour tissues from patients with CRC or CAC compared with controls. Expression of CD177 mRNA and percentages of CD177+ neutrophils were also markedly increased in tumour tissues from CRC patients compared with controls. Patients with high density of CD177+ neutrophils had better overall survival and disease-free survival compared with controls. Multivariate analyses revealed that the density of CD177+ neutrophils was an independent factor in predicting overall survival and disease-free survival. Consistently, CD177 depletion aggravated azoxymethane/dextran sodium sulphate-induced CAC in mice. Expression of Ki67 and proliferating cell nuclear antigen was increased in tumour tissues from CD177-/- mice compared with wild-type counterparts. Moreover, CD177-/- neutrophils failed to migrate in response to fMLP[AU: Please expand fMLP, DN, TNM and HIF-1a.] stimulation compared with wild-type controls. Our data indicate that CD177+ neutrophils suppress epithelial cell tumourigenesis and act as an independent factor in predicting the prognosis in patients with CRC. CD177+ neutrophils may serve as a novel therapeutic target in the treatment and predict the prognosis of CAC and CRC.

    Original languageEnglish (US)
    Pages (from-to)272-282
    Number of pages11
    JournalCarcinogenesis
    Volume39
    Issue number2
    DOIs
    StatePublished - Feb 1 2018

    Fingerprint

    Colitis
    Colorectal Neoplasms
    Neutrophils
    Epithelial Cells
    Neoplasms
    Azoxymethane
    Dextran Sulfate
    Disease-Free Survival
    Survival
    Proliferating Cell Nuclear Antigen
    Real-Time Polymerase Chain Reaction
    Flow Cytometry
    Colon
    Multivariate Analysis
    Immunohistochemistry

    ASJC Scopus subject areas

    • Cancer Research

    Cite this

    CD177+ neutrophils suppress epithelial cell tumourigenesis in colitis-associated cancer and predict good prognosis in colorectal cancer. / Zhou, Guangxi; Peng, Kangsheng; Song, Yang; Yang, Wenjing; Shu, Weigang; Yu, Tianming; Yu, Lin; Lin, Moubin; Wei, Qing; Chen, Chunqiu; Yin, Lu; Cong, Yingzi; Liu, Zhanju.

    In: Carcinogenesis, Vol. 39, No. 2, 01.02.2018, p. 272-282.

    Research output: Contribution to journalArticle

    Zhou, G, Peng, K, Song, Y, Yang, W, Shu, W, Yu, T, Yu, L, Lin, M, Wei, Q, Chen, C, Yin, L, Cong, Y & Liu, Z 2018, 'CD177+ neutrophils suppress epithelial cell tumourigenesis in colitis-associated cancer and predict good prognosis in colorectal cancer', Carcinogenesis, vol. 39, no. 2, pp. 272-282. https://doi.org/10.1093/carcin/bgx142
    Zhou, Guangxi ; Peng, Kangsheng ; Song, Yang ; Yang, Wenjing ; Shu, Weigang ; Yu, Tianming ; Yu, Lin ; Lin, Moubin ; Wei, Qing ; Chen, Chunqiu ; Yin, Lu ; Cong, Yingzi ; Liu, Zhanju. / CD177+ neutrophils suppress epithelial cell tumourigenesis in colitis-associated cancer and predict good prognosis in colorectal cancer. In: Carcinogenesis. 2018 ; Vol. 39, No. 2. pp. 272-282.
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    abstract = "Neutrophils are found to be infiltrated in tumour tissues of patients with colitis-associated cancer (CAC) and colorectal cancer (CRC), and CD177 is mainly expressed in neutrophils. In our study, expression of CD177 in tumour tissues from patients with CAC or CRC was analysed byquantitative real-time polymerase chain reaction, flow cytometry and immunohistochemistry. We recruited 378 patients with CRC, determined CD177 expression in tumours and examined its correlation with clinicopathological features. Moreover, CAC model was induced in wild-type and CD177-/- mice by azoxymethane/dextran sodium sulphate. CD177+ neutrophils were significantly increased in colon tumour tissues from patients with CRC or CAC compared with controls. Expression of CD177 mRNA and percentages of CD177+ neutrophils were also markedly increased in tumour tissues from CRC patients compared with controls. Patients with high density of CD177+ neutrophils had better overall survival and disease-free survival compared with controls. Multivariate analyses revealed that the density of CD177+ neutrophils was an independent factor in predicting overall survival and disease-free survival. Consistently, CD177 depletion aggravated azoxymethane/dextran sodium sulphate-induced CAC in mice. Expression of Ki67 and proliferating cell nuclear antigen was increased in tumour tissues from CD177-/- mice compared with wild-type counterparts. Moreover, CD177-/- neutrophils failed to migrate in response to fMLP[AU: Please expand fMLP, DN, TNM and HIF-1a.] stimulation compared with wild-type controls. Our data indicate that CD177+ neutrophils suppress epithelial cell tumourigenesis and act as an independent factor in predicting the prognosis in patients with CRC. CD177+ neutrophils may serve as a novel therapeutic target in the treatment and predict the prognosis of CAC and CRC.",
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    AU - Zhou, Guangxi

    AU - Peng, Kangsheng

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    AU - Shu, Weigang

    AU - Yu, Tianming

    AU - Yu, Lin

    AU - Lin, Moubin

    AU - Wei, Qing

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    AU - Yin, Lu

    AU - Cong, Yingzi

    AU - Liu, Zhanju

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    AB - Neutrophils are found to be infiltrated in tumour tissues of patients with colitis-associated cancer (CAC) and colorectal cancer (CRC), and CD177 is mainly expressed in neutrophils. In our study, expression of CD177 in tumour tissues from patients with CAC or CRC was analysed byquantitative real-time polymerase chain reaction, flow cytometry and immunohistochemistry. We recruited 378 patients with CRC, determined CD177 expression in tumours and examined its correlation with clinicopathological features. Moreover, CAC model was induced in wild-type and CD177-/- mice by azoxymethane/dextran sodium sulphate. CD177+ neutrophils were significantly increased in colon tumour tissues from patients with CRC or CAC compared with controls. Expression of CD177 mRNA and percentages of CD177+ neutrophils were also markedly increased in tumour tissues from CRC patients compared with controls. Patients with high density of CD177+ neutrophils had better overall survival and disease-free survival compared with controls. Multivariate analyses revealed that the density of CD177+ neutrophils was an independent factor in predicting overall survival and disease-free survival. Consistently, CD177 depletion aggravated azoxymethane/dextran sodium sulphate-induced CAC in mice. Expression of Ki67 and proliferating cell nuclear antigen was increased in tumour tissues from CD177-/- mice compared with wild-type counterparts. Moreover, CD177-/- neutrophils failed to migrate in response to fMLP[AU: Please expand fMLP, DN, TNM and HIF-1a.] stimulation compared with wild-type controls. Our data indicate that CD177+ neutrophils suppress epithelial cell tumourigenesis and act as an independent factor in predicting the prognosis in patients with CRC. CD177+ neutrophils may serve as a novel therapeutic target in the treatment and predict the prognosis of CAC and CRC.

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