CD44 expression positively correlates with Foxp3 expression and suppressive function of CD4+ Treg cells

Tie Liu, Lynn Soong, Gang Liu, Rolf König, Ashok Chopra

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Background: CD4+CD25+ regulatory T (Treg) cells develop in the thymus and can suppress T cell proliferation, modulated by Foxp3 and cytokines; however, the relevance of CD44 in Treg cell development is less clear. To address this issue, we analyzed Foxp3 expression in CD44+ Treg cells by using multiple parameters, measured the levels of the immunoregulatory cytokine interleukin (IL)-10 in various thymocyte subsets, and determined the suppressor activity in different splenic Treg cell populations. Results: Within mouse thymocytes, we detected Treg cells with two novel phenotypes, namely the CD4+CD8-CD25+CD44+ and CD4+CD8-CD25+CD44- staining features. Additional multi-parameter analyses at the single-cell and molecular levels suggested to us that CD44 expression positively correlated with Foxp3 expression in thymocytes, the production of IL-10, and Treg activity in splenic CD4+CD25+ T cells. This suppressive effect of Treg cells on T cell proliferation could be blocked by using anti-IL-10 neutralizing antibodies. In addition, CD4+CD25+CD44+ Treg cells expressed higher levels of IL-10 and were more potent in suppressing effector T cell proliferation than were CD4+CD25+CD44- cells. Conclusion: This study indicates the presence of two novel phenotypes of Treg cells in the thymus, the functional relevance of CD44 in defining Treg cell subsets, and the role of both IL-10 and Foxp3 in modulating the function of Treg cells. Reviewers: This article was reviewed by Dr. M. Lenardo, Dr. L. Klein & G. Wirnsberger (nominated by Dr. JC Zungia-Pfluker), and Dr. E.M. Shevach.

Original languageEnglish (US)
Article number1745
Pages (from-to)40
Number of pages1
JournalBiology Direct
Volume4
DOIs
StatePublished - Oct 23 2009

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T-cells
Regulatory T-Lymphocytes
Interleukin-10
Correlate
phenotype
Cell proliferation
Thymus
Interleukin
Cell
interleukin-10
antibody
cells
Cell Proliferation
T-lymphocytes
thymocytes
Thymocytes
Cytokines
Antibodies
T-Lymphocytes
cell proliferation

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology
  • Applied Mathematics
  • Modeling and Simulation
  • Ecology, Evolution, Behavior and Systematics

Cite this

CD44 expression positively correlates with Foxp3 expression and suppressive function of CD4+ Treg cells. / Liu, Tie; Soong, Lynn; Liu, Gang; König, Rolf; Chopra, Ashok.

In: Biology Direct, Vol. 4, 1745, 23.10.2009, p. 40.

Research output: Contribution to journalArticle

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