CD4- CD8- TCRα/β+ suppressor T cells demonstrated in mice 1 day after thermal injury

Ryuichi Matsuo, David Herndon, Makiko Kobayashi, Richard B. Pollard, Fujio Suzuki

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

We have described previously that CD8+ CD11b+ TCRγ/δ+ type 2 T cells (BA-type 2 T cells) and suppressor macrophages (Sup-Mφ) are generated in spleens of mice 3 to 5 days (Sup-Mφ) and 4 to 9 days (BA-type 2 T cells) after thermal injury. In the present study, an additional suppressor T cell, characterized as CD3+ CD4- CD8- TCRα/β+ T cells (double negative suppressor T cells, DN Sup-T cells), was demonstrated in mice 1 day after thermal injury. DN Sup-T cells inhibited the proliferation of lymphocytes stimulated with allogeneic cells or a lectin in a mixed lymphocyte reaction, and produced both type 1 and type 2 cytokines (interferon-γ, interleukin-2, interleukin-4, and interleukin-10) when they were stimulated in vitro with anti-CD3 monoclonal antibody. These results suggest that DN Sup-T cells express phenotypic properties similar to natural suppressor cells and cytokine-producing profiles different from type 1 (Th1 cells and CTLs) and type 2 T cells (Th2 cells and CD8+ type 2 T cells). DN Sup-T cells may play a role on the burn-associated immunosuppression appearing in the acute phase of thermally injured individuals.

Original languageEnglish (US)
Pages (from-to)635-640
Number of pages6
JournalJournal of Trauma - Injury, Infection and Critical Care
Volume42
Issue number4
StatePublished - Apr 1997

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Hot Temperature
T-Lymphocytes
Wounds and Injuries
Macrophages
Cytokines
Th2 Cells
Th1 Cells
Mixed Lymphocyte Culture Test
Burns
Lectins
Interleukin-4
Interleukin-10
Immunosuppression
Interferon-gamma
Interleukin-2
Spleen
Monoclonal Antibodies
Cell Proliferation
Lymphocytes

ASJC Scopus subject areas

  • Surgery

Cite this

CD4- CD8- TCRα/β+ suppressor T cells demonstrated in mice 1 day after thermal injury. / Matsuo, Ryuichi; Herndon, David; Kobayashi, Makiko; Pollard, Richard B.; Suzuki, Fujio.

In: Journal of Trauma - Injury, Infection and Critical Care, Vol. 42, No. 4, 04.1997, p. 635-640.

Research output: Contribution to journalArticle

Matsuo, Ryuichi ; Herndon, David ; Kobayashi, Makiko ; Pollard, Richard B. ; Suzuki, Fujio. / CD4- CD8- TCRα/β+ suppressor T cells demonstrated in mice 1 day after thermal injury. In: Journal of Trauma - Injury, Infection and Critical Care. 1997 ; Vol. 42, No. 4. pp. 635-640.
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