CD8+ type-2 T cells enhance the severity of acute herpes virus infection in mice

Kaori Ikemoto, Richard B. Pollard, Tetsuo Fukumoto, Mitsunori Morimatsu, Fujio Suzuki

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The role of CD8+ suppressor T cells in acute herpes simplex virus type 1 (HSV) infection was investigated in mice. CD8+ CD11b+ TCR-γ/δ+ suppressor T cells (HSV-STC) were demonstrated in spleens of mice infected intraperitoneally (i.p.) with HSV. When HSV-STC from mice infected with a 10 LD50 of HSV (donors) were adoptively transferred to mice 3 days after being infected with a 1 LD50 dose of HSV (recipients), the morbidity and mortality of recipients were greatly increased (mean survival time in days (MSD): 9.4 days; mortality, 100%) as compared with controls that received CD4+ T cells or a whole T-cell lysate from donors (MSD, > 19.6 days or > 19.1 days; mortality, 38% or 50%). The morbidity and mortality of mice exposed to a 1 LD50 of HSV were also increased when they were continuously treated with recombinant murine IL-4. However, the survival rate of mice exposed to a 10 LD50 of HSV increased after multiple treatments of these mice with anti-IL-4 monoclonal antibody. IL-4-producing cells were detected in a population of HSV-STC, and IL-4 was produced when these cells were cultured in the presence of UV-inactivated HSV in vitro. These results indicate that IL-4 plays an important role in the progression of acute HSV infection and, through the production of IL-4, HSV-STC may increase the severity of the acute-phase infection of HSV in mice.

Original languageEnglish (US)
Pages (from-to)63-72
Number of pages10
JournalImmunology Letters
Volume47
Issue number1-2
DOIs
StatePublished - 1995

Fingerprint

Human Herpesvirus 1
Virus Diseases
T-Lymphocytes
Interleukin-4
Lethal Dose 50
Survival Rate
Mortality
Morbidity
Cultured Cells
Spleen
Monoclonal Antibodies

Keywords

  • CD8 type-2 T cell
  • Herpes simplex virus type 1
  • IL-4
  • Suppressor T cell

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

CD8+ type-2 T cells enhance the severity of acute herpes virus infection in mice. / Ikemoto, Kaori; Pollard, Richard B.; Fukumoto, Tetsuo; Morimatsu, Mitsunori; Suzuki, Fujio.

In: Immunology Letters, Vol. 47, No. 1-2, 1995, p. 63-72.

Research output: Contribution to journalArticle

Ikemoto, Kaori ; Pollard, Richard B. ; Fukumoto, Tetsuo ; Morimatsu, Mitsunori ; Suzuki, Fujio. / CD8+ type-2 T cells enhance the severity of acute herpes virus infection in mice. In: Immunology Letters. 1995 ; Vol. 47, No. 1-2. pp. 63-72.
@article{514092144cd943ebacb5b9a6c4497810,
title = "CD8+ type-2 T cells enhance the severity of acute herpes virus infection in mice",
abstract = "The role of CD8+ suppressor T cells in acute herpes simplex virus type 1 (HSV) infection was investigated in mice. CD8+ CD11b+ TCR-γ/δ+ suppressor T cells (HSV-STC) were demonstrated in spleens of mice infected intraperitoneally (i.p.) with HSV. When HSV-STC from mice infected with a 10 LD50 of HSV (donors) were adoptively transferred to mice 3 days after being infected with a 1 LD50 dose of HSV (recipients), the morbidity and mortality of recipients were greatly increased (mean survival time in days (MSD): 9.4 days; mortality, 100{\%}) as compared with controls that received CD4+ T cells or a whole T-cell lysate from donors (MSD, > 19.6 days or > 19.1 days; mortality, 38{\%} or 50{\%}). The morbidity and mortality of mice exposed to a 1 LD50 of HSV were also increased when they were continuously treated with recombinant murine IL-4. However, the survival rate of mice exposed to a 10 LD50 of HSV increased after multiple treatments of these mice with anti-IL-4 monoclonal antibody. IL-4-producing cells were detected in a population of HSV-STC, and IL-4 was produced when these cells were cultured in the presence of UV-inactivated HSV in vitro. These results indicate that IL-4 plays an important role in the progression of acute HSV infection and, through the production of IL-4, HSV-STC may increase the severity of the acute-phase infection of HSV in mice.",
keywords = "CD8 type-2 T cell, Herpes simplex virus type 1, IL-4, Suppressor T cell",
author = "Kaori Ikemoto and Pollard, {Richard B.} and Tetsuo Fukumoto and Mitsunori Morimatsu and Fujio Suzuki",
year = "1995",
doi = "10.1016/0165-2478(95)00063-B",
language = "English (US)",
volume = "47",
pages = "63--72",
journal = "Immunology Letters",
issn = "0165-2478",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - CD8+ type-2 T cells enhance the severity of acute herpes virus infection in mice

AU - Ikemoto, Kaori

AU - Pollard, Richard B.

AU - Fukumoto, Tetsuo

AU - Morimatsu, Mitsunori

AU - Suzuki, Fujio

PY - 1995

Y1 - 1995

N2 - The role of CD8+ suppressor T cells in acute herpes simplex virus type 1 (HSV) infection was investigated in mice. CD8+ CD11b+ TCR-γ/δ+ suppressor T cells (HSV-STC) were demonstrated in spleens of mice infected intraperitoneally (i.p.) with HSV. When HSV-STC from mice infected with a 10 LD50 of HSV (donors) were adoptively transferred to mice 3 days after being infected with a 1 LD50 dose of HSV (recipients), the morbidity and mortality of recipients were greatly increased (mean survival time in days (MSD): 9.4 days; mortality, 100%) as compared with controls that received CD4+ T cells or a whole T-cell lysate from donors (MSD, > 19.6 days or > 19.1 days; mortality, 38% or 50%). The morbidity and mortality of mice exposed to a 1 LD50 of HSV were also increased when they were continuously treated with recombinant murine IL-4. However, the survival rate of mice exposed to a 10 LD50 of HSV increased after multiple treatments of these mice with anti-IL-4 monoclonal antibody. IL-4-producing cells were detected in a population of HSV-STC, and IL-4 was produced when these cells were cultured in the presence of UV-inactivated HSV in vitro. These results indicate that IL-4 plays an important role in the progression of acute HSV infection and, through the production of IL-4, HSV-STC may increase the severity of the acute-phase infection of HSV in mice.

AB - The role of CD8+ suppressor T cells in acute herpes simplex virus type 1 (HSV) infection was investigated in mice. CD8+ CD11b+ TCR-γ/δ+ suppressor T cells (HSV-STC) were demonstrated in spleens of mice infected intraperitoneally (i.p.) with HSV. When HSV-STC from mice infected with a 10 LD50 of HSV (donors) were adoptively transferred to mice 3 days after being infected with a 1 LD50 dose of HSV (recipients), the morbidity and mortality of recipients were greatly increased (mean survival time in days (MSD): 9.4 days; mortality, 100%) as compared with controls that received CD4+ T cells or a whole T-cell lysate from donors (MSD, > 19.6 days or > 19.1 days; mortality, 38% or 50%). The morbidity and mortality of mice exposed to a 1 LD50 of HSV were also increased when they were continuously treated with recombinant murine IL-4. However, the survival rate of mice exposed to a 10 LD50 of HSV increased after multiple treatments of these mice with anti-IL-4 monoclonal antibody. IL-4-producing cells were detected in a population of HSV-STC, and IL-4 was produced when these cells were cultured in the presence of UV-inactivated HSV in vitro. These results indicate that IL-4 plays an important role in the progression of acute HSV infection and, through the production of IL-4, HSV-STC may increase the severity of the acute-phase infection of HSV in mice.

KW - CD8 type-2 T cell

KW - Herpes simplex virus type 1

KW - IL-4

KW - Suppressor T cell

UR - http://www.scopus.com/inward/record.url?scp=0029088249&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029088249&partnerID=8YFLogxK

U2 - 10.1016/0165-2478(95)00063-B

DO - 10.1016/0165-2478(95)00063-B

M3 - Article

VL - 47

SP - 63

EP - 72

JO - Immunology Letters

JF - Immunology Letters

SN - 0165-2478

IS - 1-2

ER -