Cell crawling mediates collective cell migration to close undamaged epithelial gaps

Ester Anon, Xavier Serra-Picamal, Pascal Hersen, Nils C. Gauthier, Michael Sheetz, Xavier Trepat, Benoît Ladoux

Research output: Contribution to journalArticle

104 Citations (Scopus)

Abstract

Fundamental biological processes such as morphogenesis and wound healing involve the closure of epithelial gaps. Epithelial gap closure is commonly attributed either to the purse-string contraction of an intercellular actomyosin cable or to active cell migration, but the relative contribution of these two mechanisms remains unknown. Here we present a model experiment to systematically study epithelial closure in the absence of cell injury. We developed a pillar stencil approach to create well-defined gaps in terms of size and shape within an epithelial cell monolayer. Upon pillar removal, cells actively respond to the newly accessible free space by extending lamellipodia and migrating into the gap. The decrease of gap area over time is strikingly linear and shows two different regimes depending on the size of the gap. In large gaps, closure is dominated by lamellipodium-mediated cell migration. By contrast, closure of gaps smaller than 20 μm was affected by cell density and progressed independently of Rac, myosin light chain kinase, and Rho kinase, suggesting a passive physical mechanism. By changing the shape of the gap, we observed that low-curvature areas favored the appearance of lamellipodia, promoting faster closure. Altogether, our results reveal that the closure of epithelial gaps in the absence of cell injury is governed by the collective migration of cells through the activation of lamellipodium protrusion.

Original languageEnglish (US)
Pages (from-to)10891-10896
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume109
Issue number27
DOIs
StatePublished - Jul 3 2012
Externally publishedYes

Fingerprint

Pseudopodia
Cell Movement
Myosin-Light-Chain Kinase
Biological Phenomena
Actomyosin
rho-Associated Kinases
Wounds and Injuries
Morphogenesis
Wound Healing
Cell Count
Epithelial Cells

Keywords

  • Epithelial cell migration
  • Madin-Darby canine kidney cells
  • Microfabrication
  • Wound model assay

ASJC Scopus subject areas

  • General

Cite this

Cell crawling mediates collective cell migration to close undamaged epithelial gaps. / Anon, Ester; Serra-Picamal, Xavier; Hersen, Pascal; Gauthier, Nils C.; Sheetz, Michael; Trepat, Xavier; Ladoux, Benoît.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, No. 27, 03.07.2012, p. 10891-10896.

Research output: Contribution to journalArticle

Anon, Ester ; Serra-Picamal, Xavier ; Hersen, Pascal ; Gauthier, Nils C. ; Sheetz, Michael ; Trepat, Xavier ; Ladoux, Benoît. / Cell crawling mediates collective cell migration to close undamaged epithelial gaps. In: Proceedings of the National Academy of Sciences of the United States of America. 2012 ; Vol. 109, No. 27. pp. 10891-10896.
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