Cell culture and electron microscopy for identifying viruses in diseases of unknown cause

Cynthia S. Goldsmith; Thomas G. Ksiazek; Pierre E. Rollin; James A. Comer; William L. Nicholson; Teresa C.T. Peret; Dean D. Erdman; William J. Bellini; Brian H. Harcourt; Paul A. Rota; Julu Bhatnagar; Michael D. Bowen; Bobbie R. Erickson; Laura K. McMullan; Stuart T. Nichol; Wun Ju Shieh; Christopher D. Paddock; Sherif R. Zaki

doi:10.3201/eid1906.130173

Cell culture and electron microscopy for identifying viruses in diseases of unknown cause

Cynthia S. Goldsmith, Thomas G. Ksiazek, Pierre E. Rollin, James A. Comer, William L. Nicholson, Teresa C.T. Peret, Dean D. Erdman, William J. Bellini, Brian H. Harcourt, Paul A. Rota, Julu Bhatnagar, Michael D. Bowen, Bobbie R. Erickson, Laura K. McMullan, Stuart T. Nichol, Wun Ju Shieh, Christopher D. Paddock, Sherif R. Zaki

Pathology

Research output: Contribution to journal › Article › peer-review

39 Scopus citations

Abstract

During outbreaks of infectious diseases or in cases of severely ill patients, it is imperative to identify the causative agent. This report describes several events in which virus isolation and identification by electron microscopy were critical to initial recognition of the etiologic agent, which was further analyzed by additional laboratory diagnostic assays. Examples include severe acute respiratory syndrome coronavirus, and Nipah, lymphocytic choriomeningitis, West Nile, Cache Valley, and Heartland viruses. These cases illustrate the importance of the techniques of cell culture and electron microscopy in pathogen identification and recognition of emerging diseases.

Original language	English (US)
Pages (from-to)	886-891
Number of pages	6
Journal	Emerging infectious diseases
Volume	19
Issue number	6
DOIs	https://doi.org/10.3201/eid1906.130173
State	Published - Jun 2013

ASJC Scopus subject areas

Epidemiology
Microbiology (medical)
Infectious Diseases

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Goldsmith, C. S., Ksiazek, T. G., Rollin, P. E., Comer, J. A., Nicholson, W. L., Peret, T. C. T., Erdman, D. D., Bellini, W. J., Harcourt, B. H., Rota, P. A., Bhatnagar, J., Bowen, M. D., Erickson, B. R., McMullan, L. K., Nichol, S. T., Shieh, W. J., Paddock, C. D., & Zaki, S. R. (2013). Cell culture and electron microscopy for identifying viruses in diseases of unknown cause. Emerging infectious diseases, 19(6), 886-891. https://doi.org/10.3201/eid1906.130173

Goldsmith, CS, Ksiazek, TG, Rollin, PE, Comer, JA, Nicholson, WL, Peret, TCT, Erdman, DD, Bellini, WJ, Harcourt, BH, Rota, PA, Bhatnagar, J, Bowen, MD, Erickson, BR, McMullan, LK, Nichol, ST, Shieh, WJ, Paddock, CD & Zaki, SR 2013, 'Cell culture and electron microscopy for identifying viruses in diseases of unknown cause', Emerging infectious diseases, vol. 19, no. 6, pp. 886-891. https://doi.org/10.3201/eid1906.130173

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abstract = "During outbreaks of infectious diseases or in cases of severely ill patients, it is imperative to identify the causative agent. This report describes several events in which virus isolation and identification by electron microscopy were critical to initial recognition of the etiologic agent, which was further analyzed by additional laboratory diagnostic assays. Examples include severe acute respiratory syndrome coronavirus, and Nipah, lymphocytic choriomeningitis, West Nile, Cache Valley, and Heartland viruses. These cases illustrate the importance of the techniques of cell culture and electron microscopy in pathogen identification and recognition of emerging diseases.",

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AU - Peret, Teresa C.T.

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Cell culture and electron microscopy for identifying viruses in diseases of unknown cause

Abstract

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