Cell culture and electron microscopy for identifying viruses in diseases of unknown cause

Cynthia S. Goldsmith, Thomas Ksiazek, Pierre E. Rollin, James A. Comer, William L. Nicholson, Teresa C T Peret, Dean D. Erdman, William J. Bellini, Brian H. Harcourt, Paul A. Rota, Julu Bhatnagar, Michael D. Bowen, Bobbie R. Erickson, Laura K. McMullan, Stuart T. Nichol, Wun Ju Shieh, Christopher D. Paddock, Sherif R. Zaki

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

During outbreaks of infectious diseases or in cases of severely ill patients, it is imperative to identify the causative agent. This report describes several events in which virus isolation and identification by electron microscopy were critical to initial recognition of the etiologic agent, which was further analyzed by additional laboratory diagnostic assays. Examples include severe acute respiratory syndrome coronavirus, and Nipah, lymphocytic choriomeningitis, West Nile, Cache Valley, and Heartland viruses. These cases illustrate the importance of the techniques of cell culture and electron microscopy in pathogen identification and recognition of emerging diseases.

Original languageEnglish (US)
Pages (from-to)886-891
Number of pages6
JournalEmerging Infectious Diseases
Volume19
Issue number6
DOIs
StatePublished - Jun 2013

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Virus Diseases
Electron Microscopy
Bunyamwera virus
Lymphocytic Choriomeningitis
Cell Culture Techniques
Severe Acute Respiratory Syndrome
Coronavirus
Disease Outbreaks
Viruses

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases
  • Epidemiology

Cite this

Goldsmith, C. S., Ksiazek, T., Rollin, P. E., Comer, J. A., Nicholson, W. L., Peret, T. C. T., ... Zaki, S. R. (2013). Cell culture and electron microscopy for identifying viruses in diseases of unknown cause. Emerging Infectious Diseases, 19(6), 886-891. https://doi.org/10.3201/eid1906.130173

Cell culture and electron microscopy for identifying viruses in diseases of unknown cause. / Goldsmith, Cynthia S.; Ksiazek, Thomas; Rollin, Pierre E.; Comer, James A.; Nicholson, William L.; Peret, Teresa C T; Erdman, Dean D.; Bellini, William J.; Harcourt, Brian H.; Rota, Paul A.; Bhatnagar, Julu; Bowen, Michael D.; Erickson, Bobbie R.; McMullan, Laura K.; Nichol, Stuart T.; Shieh, Wun Ju; Paddock, Christopher D.; Zaki, Sherif R.

In: Emerging Infectious Diseases, Vol. 19, No. 6, 06.2013, p. 886-891.

Research output: Contribution to journalArticle

Goldsmith, CS, Ksiazek, T, Rollin, PE, Comer, JA, Nicholson, WL, Peret, TCT, Erdman, DD, Bellini, WJ, Harcourt, BH, Rota, PA, Bhatnagar, J, Bowen, MD, Erickson, BR, McMullan, LK, Nichol, ST, Shieh, WJ, Paddock, CD & Zaki, SR 2013, 'Cell culture and electron microscopy for identifying viruses in diseases of unknown cause', Emerging Infectious Diseases, vol. 19, no. 6, pp. 886-891. https://doi.org/10.3201/eid1906.130173
Goldsmith, Cynthia S. ; Ksiazek, Thomas ; Rollin, Pierre E. ; Comer, James A. ; Nicholson, William L. ; Peret, Teresa C T ; Erdman, Dean D. ; Bellini, William J. ; Harcourt, Brian H. ; Rota, Paul A. ; Bhatnagar, Julu ; Bowen, Michael D. ; Erickson, Bobbie R. ; McMullan, Laura K. ; Nichol, Stuart T. ; Shieh, Wun Ju ; Paddock, Christopher D. ; Zaki, Sherif R. / Cell culture and electron microscopy for identifying viruses in diseases of unknown cause. In: Emerging Infectious Diseases. 2013 ; Vol. 19, No. 6. pp. 886-891.
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