Cell migration does not produce membrane flow

Dennis F. Kucik, Elliot L. Elson, Michael Sheetz

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

We have previously reported that rearward migration of surface particles on slowly moving cells is not driven by membrane flow (Sheetz, M. P., S. Turney, H. Qian, and E. L. Elson. 1989. Nature (Lond.). 340:284-288) and recent photobleaching measurements have ruled out any rapid rearward lipid flow (Lee, J., M. Gustafsson, D. E. Magnussen, and K. Jacobson. 1990. Science (Wash. DC.) 247:1229-1233). It was not possible, however, to conclude from those studies that a slower or tank-tread membrane lipid flow does not occur. Therefore, we have used the technology of single particle tracking to examine the movements of diffusing particles on rapidly locomoting fish keratocytes where the membrane current is likely to be greatest. The keratocytes had a smooth lamellipodial surface on which bound Con A-coated gold particles were observed either to track toward the nuclear region (velocity of 0.35 ± 0.15 μm/s) or to diffuse randomly (apparent diffusion coefficient of [3.5 ± 2.0] × 10-2 cm2/s). We detected no systematic drift relative to the cell edge of particles undergoing random diffusion even after the cell had moved many micrometers. The average net particle displacement was 0.01 ± 2.7% of the cell displacement. These results strongly suggest that neither the motions of membrane proteins driven by the cytoskeleton nor other possible factors produce a bulk flow of membrane lipid.

Original languageEnglish (US)
Pages (from-to)1617-1622
Number of pages6
JournalJournal of Cell Biology
Volume111
Issue number4
DOIs
StatePublished - Jan 1 1990
Externally publishedYes

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Cell Movement
Membranes
Membrane Lipids
Photobleaching
Cytoskeleton
Membrane Proteins
Fishes
Technology
Lipids

ASJC Scopus subject areas

  • Cell Biology

Cite this

Cell migration does not produce membrane flow. / Kucik, Dennis F.; Elson, Elliot L.; Sheetz, Michael.

In: Journal of Cell Biology, Vol. 111, No. 4, 01.01.1990, p. 1617-1622.

Research output: Contribution to journalArticle

Kucik, Dennis F. ; Elson, Elliot L. ; Sheetz, Michael. / Cell migration does not produce membrane flow. In: Journal of Cell Biology. 1990 ; Vol. 111, No. 4. pp. 1617-1622.
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