Cell spreading and lamellipodial extension rate is regulated by membrane tension

Drazen Raucher, Michael Sheetz

Research output: Contribution to journalArticle

307 Citations (Scopus)

Abstract

Cell spreading and motility require the extension of the plasma membrane in association with the assembly of actin. In vitro, extension must overcome resistance from tension within the plasma membrane. We report here that the addition of either amphiphilic compounds or fluorescent lipids that expanded the plasma membrane increased the rate of cell spreading and lamellipodial extension, stimulated new lamellipodial extensions, and caused a decrease in the apparent membrane tension. Further, in PDGF-stimulated motility, the increase in the lamellipodial extension rate was associated with a decrease in the apparent membrane tension and decreased membrane-cytoskeleton adhesion through phosphatidylinositol diphosphate hydrolysis. Conversely, when membrane tension was increased by osmotically swelling cells, the extension rate decreased. Therefore, we suggest that the lamellipodial extension process can be activated by a physical signal (perhaps secondarily), and the rate of extension is directly dependent upon the tension in the plasma membrane. Quantitative analysis shows that the lamellipodial extension rate is inversely correlated with the apparent membrane tension. These studies describe a physical chemical mechanism involving changes in membrane- cytoskeleton adhesion through phosphatidylinositol 4,5-biphosphate-protein interactions for modulating and stimulating the biochemical processes that power lamellipodial extension.

Original languageEnglish (US)
Pages (from-to)127-136
Number of pages10
JournalJournal of Cell Biology
Volume148
Issue number1
DOIs
StatePublished - Jan 10 2000
Externally publishedYes

Fingerprint

Membranes
Cell Membrane
Cytoskeleton
Biochemical Phenomena
Phosphatidylinositol 4,5-Diphosphate
Diphosphates
Phosphatidylinositols
Cell Movement
Actins
Hydrolysis
Lipids
Proteins

Keywords

  • Cell spreading
  • Laser optical tweezers
  • Membrane expansion
  • Membrane tether
  • Membrane-cytoskeleton adhesion

ASJC Scopus subject areas

  • Cell Biology

Cite this

Cell spreading and lamellipodial extension rate is regulated by membrane tension. / Raucher, Drazen; Sheetz, Michael.

In: Journal of Cell Biology, Vol. 148, No. 1, 10.01.2000, p. 127-136.

Research output: Contribution to journalArticle

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