Cell spreading as a hydrodynamic process

M. A. Fardin, O. M. Rossier, P. Rangamani, P. D. Avigan, N. C. Gauthier, W. Vonnegut, A. Mathur, J. Hone, R. Iyengar, Michael Sheetz

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Many cell types have the ability to move themselves by crawling on extra-cellular matrices. Although cell motility is governed by actin and myosin filament assembly, the pattern of the movement follows the physical properties of the network ensemble average. The first step of motility, cell spreading on matrix substrates, involves a transition from round cells in suspension to polarized cells on substrates. Here we show that the spreading dynamics on 2D surfaces can be described as a hydrodynamic process. In particular, we show that the transition from isotropic spreading at early time to anisotropic spreading is reminiscent of the fingering instability observed in many spreading fluids. During cell spreading, the main driving force is the polymerization of actin filaments that push the membrane forward. From the equilibrium between the membrane force and the cytoskeleton, we derive a first order expression of the polymerization stress that reproduces the observed behavior. Our model also allows an interpretation of the effects of pharmacological agents altering the polymerization of actin. In particular we describe the influence of Cytochalasin D on the nucleation of the fingering instability.

Original languageEnglish (US)
Pages (from-to)4788-4799
Number of pages12
JournalSoft Matter
Volume6
Issue number19
DOIs
StatePublished - Oct 7 2010
Externally publishedYes

Fingerprint

Actins
Hydrodynamics
hydrodynamics
Polymerization
cells
Membranes
Cytochalasin D
locomotion
polymerization
Substrates
Myosins
filaments
Suspensions
Nucleation
Physical properties
membranes
myosins
Fluids
matrices
assembly

ASJC Scopus subject areas

  • Chemistry(all)
  • Condensed Matter Physics

Cite this

Fardin, M. A., Rossier, O. M., Rangamani, P., Avigan, P. D., Gauthier, N. C., Vonnegut, W., ... Sheetz, M. (2010). Cell spreading as a hydrodynamic process. Soft Matter, 6(19), 4788-4799. https://doi.org/10.1039/c0sm00252f

Cell spreading as a hydrodynamic process. / Fardin, M. A.; Rossier, O. M.; Rangamani, P.; Avigan, P. D.; Gauthier, N. C.; Vonnegut, W.; Mathur, A.; Hone, J.; Iyengar, R.; Sheetz, Michael.

In: Soft Matter, Vol. 6, No. 19, 07.10.2010, p. 4788-4799.

Research output: Contribution to journalArticle

Fardin, MA, Rossier, OM, Rangamani, P, Avigan, PD, Gauthier, NC, Vonnegut, W, Mathur, A, Hone, J, Iyengar, R & Sheetz, M 2010, 'Cell spreading as a hydrodynamic process', Soft Matter, vol. 6, no. 19, pp. 4788-4799. https://doi.org/10.1039/c0sm00252f
Fardin MA, Rossier OM, Rangamani P, Avigan PD, Gauthier NC, Vonnegut W et al. Cell spreading as a hydrodynamic process. Soft Matter. 2010 Oct 7;6(19):4788-4799. https://doi.org/10.1039/c0sm00252f
Fardin, M. A. ; Rossier, O. M. ; Rangamani, P. ; Avigan, P. D. ; Gauthier, N. C. ; Vonnegut, W. ; Mathur, A. ; Hone, J. ; Iyengar, R. ; Sheetz, Michael. / Cell spreading as a hydrodynamic process. In: Soft Matter. 2010 ; Vol. 6, No. 19. pp. 4788-4799.
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