Cellular autophagocytosis induced by deprivation of serum and amino acids in HeLa cells

J. S. Mitchener, J. D. Shelburne, W. D. Bradford, H. K. Hawkins

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

Morphologic alterations in HeLa cells deprived of serum and amino acids were studied by light and electron microscopy. After complete deprivation for 1, 3, or 6 hr, many cells contained autophagic vacuoles, enclosing clearly recognizable cell organelles. The proportion of cells showing autophagy rose from 4 to 37% in the first 3 hr of deprivation. Acid phosphatase reaction product was localized within many of the autophagic vacuoles by a modified Gomori method. After 12 or 24 hr of deprivation, many membranous residual bodies were noted within the starved HeLa cells. These electron microscopic studies indicate that complete deprivation of serum and amino acids provides a useful model for the further study of cellular autophagocytosis.

Original languageEnglish (US)
Pages (from-to)485-498
Number of pages14
JournalAmerican Journal of Pathology
Volume83
Issue number3
StatePublished - 1976
Externally publishedYes

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Autophagy
HeLa Cells
Vacuoles
Amino Acids
Serum
Acid Phosphatase
Organelles
Electron Microscopy
Electrons
Light

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Mitchener, J. S., Shelburne, J. D., Bradford, W. D., & Hawkins, H. K. (1976). Cellular autophagocytosis induced by deprivation of serum and amino acids in HeLa cells. American Journal of Pathology, 83(3), 485-498.

Cellular autophagocytosis induced by deprivation of serum and amino acids in HeLa cells. / Mitchener, J. S.; Shelburne, J. D.; Bradford, W. D.; Hawkins, H. K.

In: American Journal of Pathology, Vol. 83, No. 3, 1976, p. 485-498.

Research output: Contribution to journalArticle

Mitchener, JS, Shelburne, JD, Bradford, WD & Hawkins, HK 1976, 'Cellular autophagocytosis induced by deprivation of serum and amino acids in HeLa cells', American Journal of Pathology, vol. 83, no. 3, pp. 485-498.
Mitchener, J. S. ; Shelburne, J. D. ; Bradford, W. D. ; Hawkins, H. K. / Cellular autophagocytosis induced by deprivation of serum and amino acids in HeLa cells. In: American Journal of Pathology. 1976 ; Vol. 83, No. 3. pp. 485-498.
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