Cellular immune response to acetylcholine receptors in murine experimental autoimmune myasthenia gravis: Inhibition with monoclonal anti-I-A antibodies

Premkumar Christadoss; Jon Lindstrom; Norman Talal

doi:10.1016/0008-8749(83)90205-8

Cellular immune response to acetylcholine receptors in murine experimental autoimmune myasthenia gravis: Inhibition with monoclonal anti-I-A antibodies

Premkumar Christadoss, Jon Lindstrom, Norman Talal

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

Gene(s) at the I-A subregion of the murine major histocompatibility complex influence susceptibility to experimental autoimmune myasthenia gravis. C57B1/6 mice immunized with acetylcholine receptors (AChR) in complete Freund's adjuvant demonstrated cellular and humoral immune responses to AChR. They developed muscle weakness characteristic of myasthenia gravis and demonstrated a reduction in the muscle AChR content. The kinetics of AChR-specific lymphocyte proliferation generally correlate with anti-AChR antibody response. AChR-specific lymphocyte proliferation was also observed in C57B1/6 splenocytes after secondary immunization with AChR. The in vitro cellular reactivity to AChR in experimental autoimmune myasthenia gravis (EAMG) mice (C57B1/6) was suppressed by monoclonal anti-I-A^b antibodies directed against private (Ia20) or public (Ia8) specificities, suggesting a critical role for these Ia determinants in the cellular immune response to AChR in murine EAMG.

Original language	English (US)
Pages (from-to)	1-8
Number of pages	8
Journal	Cellular Immunology
Volume	81
Issue number	1
DOIs	https://doi.org/10.1016/0008-8749(83)90205-8
State	Published - Oct 1 1983
Externally published	Yes

ASJC Scopus subject areas

Immunology

Access to Document

10.1016/0008-8749(83)90205-8

Cite this

@article{acfb5a53ef1b4901b10bde76d8df3da0,

title = "Cellular immune response to acetylcholine receptors in murine experimental autoimmune myasthenia gravis: Inhibition with monoclonal anti-I-A antibodies",

abstract = "Gene(s) at the I-A subregion of the murine major histocompatibility complex influence susceptibility to experimental autoimmune myasthenia gravis. C57B1/6 mice immunized with acetylcholine receptors (AChR) in complete Freund's adjuvant demonstrated cellular and humoral immune responses to AChR. They developed muscle weakness characteristic of myasthenia gravis and demonstrated a reduction in the muscle AChR content. The kinetics of AChR-specific lymphocyte proliferation generally correlate with anti-AChR antibody response. AChR-specific lymphocyte proliferation was also observed in C57B1/6 splenocytes after secondary immunization with AChR. The in vitro cellular reactivity to AChR in experimental autoimmune myasthenia gravis (EAMG) mice (C57B1/6) was suppressed by monoclonal anti-I-Ab antibodies directed against private (Ia20) or public (Ia8) specificities, suggesting a critical role for these Ia determinants in the cellular immune response to AChR in murine EAMG.",

author = "Premkumar Christadoss and Jon Lindstrom and Norman Talal",

note = "Funding Information: {\textquoteright} Supported by a grant from the Muscular Dystrophy Association and the Veterans Administration. *To whom correspondence should be addressed. Department of Medicine, Division of Clinical Immunology, The University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Dr., San Antonio, Tex. 78284. {\textquoteleft}Abbreviations used: AChR, acetylcholine receptors; MC, myasthenia gravis; EAMG, experimental autoimmune myasthenia gravis; M$, macrophages; B6, C57Bl/6; CFA, complete Freund{\textquoteright}s adjuvant; MEPP, miniature end plate potential. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.",

year = "1983",

month = oct,

day = "1",

doi = "10.1016/0008-8749(83)90205-8",

language = "English (US)",

volume = "81",

pages = "1--8",

journal = "Cellular Immunology",

issn = "0008-8749",

publisher = "Academic Press Inc.",

number = "1",

}

TY - JOUR

T1 - Cellular immune response to acetylcholine receptors in murine experimental autoimmune myasthenia gravis

T2 - Inhibition with monoclonal anti-I-A antibodies

AU - Christadoss, Premkumar

AU - Lindstrom, Jon

AU - Talal, Norman

N1 - Funding Information: ’ Supported by a grant from the Muscular Dystrophy Association and the Veterans Administration. *To whom correspondence should be addressed. Department of Medicine, Division of Clinical Immunology, The University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Dr., San Antonio, Tex. 78284. ‘Abbreviations used: AChR, acetylcholine receptors; MC, myasthenia gravis; EAMG, experimental autoimmune myasthenia gravis; M$, macrophages; B6, C57Bl/6; CFA, complete Freund’s adjuvant; MEPP, miniature end plate potential. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.

PY - 1983/10/1

Y1 - 1983/10/1

N2 - Gene(s) at the I-A subregion of the murine major histocompatibility complex influence susceptibility to experimental autoimmune myasthenia gravis. C57B1/6 mice immunized with acetylcholine receptors (AChR) in complete Freund's adjuvant demonstrated cellular and humoral immune responses to AChR. They developed muscle weakness characteristic of myasthenia gravis and demonstrated a reduction in the muscle AChR content. The kinetics of AChR-specific lymphocyte proliferation generally correlate with anti-AChR antibody response. AChR-specific lymphocyte proliferation was also observed in C57B1/6 splenocytes after secondary immunization with AChR. The in vitro cellular reactivity to AChR in experimental autoimmune myasthenia gravis (EAMG) mice (C57B1/6) was suppressed by monoclonal anti-I-Ab antibodies directed against private (Ia20) or public (Ia8) specificities, suggesting a critical role for these Ia determinants in the cellular immune response to AChR in murine EAMG.

AB - Gene(s) at the I-A subregion of the murine major histocompatibility complex influence susceptibility to experimental autoimmune myasthenia gravis. C57B1/6 mice immunized with acetylcholine receptors (AChR) in complete Freund's adjuvant demonstrated cellular and humoral immune responses to AChR. They developed muscle weakness characteristic of myasthenia gravis and demonstrated a reduction in the muscle AChR content. The kinetics of AChR-specific lymphocyte proliferation generally correlate with anti-AChR antibody response. AChR-specific lymphocyte proliferation was also observed in C57B1/6 splenocytes after secondary immunization with AChR. The in vitro cellular reactivity to AChR in experimental autoimmune myasthenia gravis (EAMG) mice (C57B1/6) was suppressed by monoclonal anti-I-Ab antibodies directed against private (Ia20) or public (Ia8) specificities, suggesting a critical role for these Ia determinants in the cellular immune response to AChR in murine EAMG.

UR - http://www.scopus.com/inward/record.url?scp=0020601994&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020601994&partnerID=8YFLogxK

U2 - 10.1016/0008-8749(83)90205-8

DO - 10.1016/0008-8749(83)90205-8

M3 - Article

C2 - 6577968

AN - SCOPUS:0020601994

SN - 0008-8749

VL - 81

SP - 1

EP - 8

JO - Cellular Immunology

JF - Cellular Immunology

IS - 1

ER -

Cellular immune response to acetylcholine receptors in murine experimental autoimmune myasthenia gravis: Inhibition with monoclonal anti-I-A antibodies

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this