Cellular localization of Saint Louis encephalitis virus replication

I. A. Brawner, M. D. Trousdale, D. W. Trent

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Replication of Saint Louis encephalitis (SLE) virus was inhibited when PS cells were treated with actinomycin D, daunomycin or cordycepin during the first 9 hr after infection. Autoradiography of SLE virus-infected pulse labelled cells demonstrated that viral RNA synthesis is localized within the nuclear area. Nuclei purified from cells after 12 hr of infection contained heterogeneous 20 S to 26 S viral RNA but no SLE virus genome sized 43S RNA. Later during infection, nuclei isolated from infected cells contained large amounts of 43 S and 20 S to 26 S RNAs. The 43 S viral RNA present in cells late in infection could not be removed with 1% Tween 80: Nonidet P 40. Purified nuclei isolated from cells early in infection supported the synthesis of 43 S virion RNA in the absence of cytoplasmic factors. The cytoplasmic membrane fraction prepared from cells early in infection contained heterogeneous 10 S to 26 S RNA species; later during infection these membranes contained viral 43 S, 26 S to 30 S and 4 S RNA. These results suggest that the nucleus is an important site of early viral synthesis.

Original languageEnglish
Pages (from-to)284-294
Number of pages11
JournalActa Virologica
Volume23
Issue number4
StatePublished - 1979

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St. Louis Encephalitis Viruses
Virus Replication
Infection
RNA
Viral RNA
Cell Nucleus
Daunorubicin
Polysorbates
Dactinomycin
Autoradiography
Virion
Cell Membrane
Genome
Membranes

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Brawner, I. A., Trousdale, M. D., & Trent, D. W. (1979). Cellular localization of Saint Louis encephalitis virus replication. Acta Virologica, 23(4), 284-294.

Cellular localization of Saint Louis encephalitis virus replication. / Brawner, I. A.; Trousdale, M. D.; Trent, D. W.

In: Acta Virologica, Vol. 23, No. 4, 1979, p. 284-294.

Research output: Contribution to journalArticle

Brawner, IA, Trousdale, MD & Trent, DW 1979, 'Cellular localization of Saint Louis encephalitis virus replication', Acta Virologica, vol. 23, no. 4, pp. 284-294.
Brawner IA, Trousdale MD, Trent DW. Cellular localization of Saint Louis encephalitis virus replication. Acta Virologica. 1979;23(4):284-294.
Brawner, I. A. ; Trousdale, M. D. ; Trent, D. W. / Cellular localization of Saint Louis encephalitis virus replication. In: Acta Virologica. 1979 ; Vol. 23, No. 4. pp. 284-294.
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