Cellular localization of Saint Louis encephalitis virus replication

I. A. Brawner, M. D. Trousdale, D. W. Trent

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


Replication of Saint Louis encephalitis (SLE) virus was inhibited when PS cells were treated with actinomycin D, daunomycin or cordycepin during the first 9 hr after infection. Autoradiography of SLE virus-infected pulse labelled cells demonstrated that viral RNA synthesis is localized within the nuclear area. Nuclei purified from cells after 12 hr of infection contained heterogeneous 20 S to 26 S viral RNA but no SLE virus genome sized 43S RNA. Later during infection, nuclei isolated from infected cells contained large amounts of 43 S and 20 S to 26 S RNAs. The 43 S viral RNA present in cells late in infection could not be removed with 1% Tween 80: Nonidet P 40. Purified nuclei isolated from cells early in infection supported the synthesis of 43 S virion RNA in the absence of cytoplasmic factors. The cytoplasmic membrane fraction prepared from cells early in infection contained heterogeneous 10 S to 26 S RNA species; later during infection these membranes contained viral 43 S, 26 S to 30 S and 4 S RNA. These results suggest that the nucleus is an important site of early viral synthesis.

Original languageEnglish (US)
Pages (from-to)284-294
Number of pages11
JournalActa virologica
Issue number4
StatePublished - 1979
Externally publishedYes

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases


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