Central nervous system DNA fragmentation induced by the inhibition of nuclear factor kappa B

Giulio Taglialatela, Joel A. Kaufmann, Angie Trevino, J. Regino Perez-Polo

    Research output: Contribution to journalArticlepeer-review

    56 Scopus citations

    Abstract

    AGEING of the central nervous system (CNS) is characterized by a progressive apoptotic loss of neurons that may be in part due to impaired neurotrophin signaling mediated by such elements as the transcription factor nuclear factor kappa B (NFκB). To support this hypothesis, we inhibited nuclear translocation of NFκB in vivo by injecting a proteasome inhibitor (PSI) directly in the CNS lateral ventricle of rats and then measured fragmented DNA in various CNS areas as an index of ongoing apoptosis. Our results show that after PSI injection there was a significant inhibition of NFκB activity in vivo that resulted in the appearance of fragmented (apoptotic) DNA in the CNS of rats. These results suggest that alteration of NFκB may cause apoptotic cell death in the rat CNS during ageing.

    Original languageEnglish (US)
    Pages (from-to)489-493
    Number of pages5
    JournalNeuroReport
    Volume9
    Issue number3
    DOIs
    StatePublished - Feb 16 1998

    Keywords

    • Ageing
    • Apoptosis
    • CNS
    • NFκB
    • Neurotrophin
    • Transcription factors

    ASJC Scopus subject areas

    • Neuroscience(all)

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