Centrifugal force stretcher a new of in vitro mechanical cell stimulator.

Yu Tao Xi, Xiao Jun Bai, Geru Wu, Ai Qun Ma

Research output: Contribution to journalArticle

Abstract

A number of mechanical cell stimulators have been used to study the effect of mechanical stimulation on cells in vitro. But the efficiency of these devices is not fully desirable. We recently developed a new device for mechanical cell stimulation, the centrifugal force stretcher, and compared its efficacy with that of the traditional Flexercell Strain Unit. When the mechanical stretcher circumrotates with certain speed, cardiac myocytes attached on the plate are stretched and elongated by centrifugal force. Neonatal rat cardiac myocytes were isolated by enzymatic dissociation from the hearts of 3~5 d old Sprague Dawley rats, and were mechanically stimulated by traditional 20% stretch and 180 r/min centrifugal force for 12 and 24 h. The effects of mechanical stimulation on the hypertrophic response of neonatal rat cardiac myocytes and production of angiotensin II (Ang II) were examined. Compared with the non-stretch group, the radioactivity of (3)H-leucine incorporated into the stretch-stimulated cardiac myocytes in the centrifugal force stretch group was significantly higher [(1295.17+/-51.19) vs (1122.67+/-51.63) in 12 h; (1447.5+/-35.96) vs (1210.67+/-90.92) in 24 h, P<0.05]. Ang II was also dramatically increased by 128% in 12 h (P<0.05) and 139% in 24 h (P<0.01). After the myocytes was stretched for 24 h, the LDH level in the medium in the Flexercell Strain Unit group was significantly higher than that in the centrifugal force group [(14.5+/-8.7) U/L vs (7.8+/-4.3) U/L, P<0.05]. The centrifugal force stretcher is a new and improved mechanical cell stimulator with the same effects on the protein synthesis and Ang II secretion of the cardiac myocytes, and the damage to the cells bronght by this stimulator is relatively slighter in comparison with the Flexercell Strain Unit.

Original languageEnglish (US)
Pages (from-to)419-423
Number of pages5
JournalSheng li xue bao : [Acta physiologica Sinica].
Volume56
Issue number3
StatePublished - Jun 25 2004
Externally publishedYes

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Stretchers
Cardiac Myocytes
Angiotensin II
Equipment and Supplies
Leucine
Muscle Cells
Radioactivity
Sprague Dawley Rats
Cell Count
In Vitro Techniques
Proteins

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Centrifugal force stretcher a new of in vitro mechanical cell stimulator. / Xi, Yu Tao; Bai, Xiao Jun; Wu, Geru; Ma, Ai Qun.

In: Sheng li xue bao : [Acta physiologica Sinica]., Vol. 56, No. 3, 25.06.2004, p. 419-423.

Research output: Contribution to journalArticle

Xi, Yu Tao ; Bai, Xiao Jun ; Wu, Geru ; Ma, Ai Qun. / Centrifugal force stretcher a new of in vitro mechanical cell stimulator. In: Sheng li xue bao : [Acta physiologica Sinica]. 2004 ; Vol. 56, No. 3. pp. 419-423.
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abstract = "A number of mechanical cell stimulators have been used to study the effect of mechanical stimulation on cells in vitro. But the efficiency of these devices is not fully desirable. We recently developed a new device for mechanical cell stimulation, the centrifugal force stretcher, and compared its efficacy with that of the traditional Flexercell Strain Unit. When the mechanical stretcher circumrotates with certain speed, cardiac myocytes attached on the plate are stretched and elongated by centrifugal force. Neonatal rat cardiac myocytes were isolated by enzymatic dissociation from the hearts of 3~5 d old Sprague Dawley rats, and were mechanically stimulated by traditional 20{\%} stretch and 180 r/min centrifugal force for 12 and 24 h. The effects of mechanical stimulation on the hypertrophic response of neonatal rat cardiac myocytes and production of angiotensin II (Ang II) were examined. Compared with the non-stretch group, the radioactivity of (3)H-leucine incorporated into the stretch-stimulated cardiac myocytes in the centrifugal force stretch group was significantly higher [(1295.17+/-51.19) vs (1122.67+/-51.63) in 12 h; (1447.5+/-35.96) vs (1210.67+/-90.92) in 24 h, P<0.05]. Ang II was also dramatically increased by 128{\%} in 12 h (P<0.05) and 139{\%} in 24 h (P<0.01). After the myocytes was stretched for 24 h, the LDH level in the medium in the Flexercell Strain Unit group was significantly higher than that in the centrifugal force group [(14.5+/-8.7) U/L vs (7.8+/-4.3) U/L, P<0.05]. The centrifugal force stretcher is a new and improved mechanical cell stimulator with the same effects on the protein synthesis and Ang II secretion of the cardiac myocytes, and the damage to the cells bronght by this stimulator is relatively slighter in comparison with the Flexercell Strain Unit.",
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