Ceruloplasmin gene expression in human cancer cells

Satya P. Kunapuli, Harbans Singh, Pomila Singh, Ashok Kumar

Research output: Contribution to journalArticle

34 Scopus citations

Abstract

The copper transport protein, ceruloplasmin, is suggested to have a role in cancer since it is involved in angiogenesis and neovascularization. In order to understand the role of ceruloplasmin in malignant cells, we have recently isolated and sequenced a human ceruloplasmin cDNA clone. In the present study, we have investigated the ceruloplasmin gene expression in human colon and breast cancer cell lines. The poly (A)+ RNA from human colon (WiDr) and human breast (MCF-7) cancer cell lines was analyzed for the presence of ceruloplasmin mRNA. The Northern blot analysis revealed the presence of a 3.7 kb band of ceruloplasmin mRNA in these cell lines. Dot blot analysis revealed that ceruloplasmin mRNA is at least three fold more abundanr in tumor cells as compared to normal rat liver.

Original languageEnglish (US)
Pages (from-to)2225-2228
Number of pages4
JournalLife Sciences
Volume40
Issue number23
DOIs
StatePublished - Jun 8 1987

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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