Cervical microbiota in women with preterm prelabor rupture of membranes

Marian Kacerovsky, Filip Vrbacky, Radka Kutova, Lenka Pliskova, Ctirad Andrys, Ivana Musilova, Ramkumar Menon, Ronald Lamont, Jana Nekvindova

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Objective: To analyze the cervical microbiota in women with preterm prelabor rupture of membranes (PPROM) by pyrosequencing and to document associations between cervical microbiota, cervical inflammatory response, microbial invasion of the amniotic cavity (MIAC), histological chorioamnionitis, and intraamniotic infection (IAI). Study Design: Sixty-one women with singleton pregnancies complicated by PPROM were included in the study. Specimens of cervical and amniotic fluid were collected on admission. The cervical microbiota was assessed by 16S rRNA gene sequencing by pyrosequencing. Interleukin (IL)-6 concentration in the cervical fluid and amniotic fluid was measured by ELISA and lateral flow immunoassay, respectively. Results: Four bacterial community state types [CST I (Lactobacillus crispatus dominated), CST III (Lactobacillus iners dominated), CST IV-A (non-Lactobacillus bacteria dominated), and CST IV-B (Gardnerella vaginalis and Sneathia sanguinegens dominated)] were observed in the cervical microbiota of women with PPROM. Cervical fluid IL-6 concentrations differed between CSTs (CST I = 145 pg/mL, CST III = 166 pg/mL, CST IV-A = 420 pg/mL, and CST IV-B = 322 pg/mL; p = 0.004). There were also differences in the rates of MIAC, of both MIAC and histological chorioamnionitis, and of IAI among CSTs. No difference in the rate of histological chorioamnionitis was found among CSTs. Conclusions: The cervical microbiota in PPROM women in this study was characterized by four CSTs. The presence of non-Lactobacillus CSTs was associated with a strong cervical inflammatory response and higher rates of MIAC, both MIAC and histological chorioamnionitis, and IAI representing a PPROM subtype with pronounced inflammation. CST I represents the dominant type of PPROM with a low rate of MIAC, IAI, and the combination of MIAC and histological chorioamnionitis.

Original languageEnglish (US)
Article numbere0126884
JournalPLoS One
Volume10
Issue number5
DOIs
StatePublished - May 20 2015

Fingerprint

Microbiota
Chorioamnionitis
Rupture
Membranes
amniotic fluid
inflammation
Fluids
interleukin-6
infection
Amniotic Fluid
Infection
Gardnerella vaginalis
Lactobacillus crispatus
Interleukin-6
immunoassays
bacterial communities
Lactobacillus
rRNA Genes
Immunoassay
experimental design

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Kacerovsky, M., Vrbacky, F., Kutova, R., Pliskova, L., Andrys, C., Musilova, I., ... Nekvindova, J. (2015). Cervical microbiota in women with preterm prelabor rupture of membranes. PLoS One, 10(5), [e0126884]. https://doi.org/10.1371/journal.pone.0126884

Cervical microbiota in women with preterm prelabor rupture of membranes. / Kacerovsky, Marian; Vrbacky, Filip; Kutova, Radka; Pliskova, Lenka; Andrys, Ctirad; Musilova, Ivana; Menon, Ramkumar; Lamont, Ronald; Nekvindova, Jana.

In: PLoS One, Vol. 10, No. 5, e0126884, 20.05.2015.

Research output: Contribution to journalArticle

Kacerovsky, M, Vrbacky, F, Kutova, R, Pliskova, L, Andrys, C, Musilova, I, Menon, R, Lamont, R & Nekvindova, J 2015, 'Cervical microbiota in women with preterm prelabor rupture of membranes', PLoS One, vol. 10, no. 5, e0126884. https://doi.org/10.1371/journal.pone.0126884
Kacerovsky M, Vrbacky F, Kutova R, Pliskova L, Andrys C, Musilova I et al. Cervical microbiota in women with preterm prelabor rupture of membranes. PLoS One. 2015 May 20;10(5). e0126884. https://doi.org/10.1371/journal.pone.0126884
Kacerovsky, Marian ; Vrbacky, Filip ; Kutova, Radka ; Pliskova, Lenka ; Andrys, Ctirad ; Musilova, Ivana ; Menon, Ramkumar ; Lamont, Ronald ; Nekvindova, Jana. / Cervical microbiota in women with preterm prelabor rupture of membranes. In: PLoS One. 2015 ; Vol. 10, No. 5.
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