cGMP-dependent and -independent angiogenesis-related properties of nitric oxide

Anastasia Pyriochou, Theodoros Vassilakopoulos, Zongmin Zhou, Andreas Papapetropoulos

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Nitric oxide exerts a stimulatory role during postnatal angiogenesis. Although soluble guanylyl cyclase (sGC) mediates many of the effects of nitric oxide (NO) in the vascular system, the contribution of cGMP-dependent vs cGMP-independent pathways in NO-induced angiogenesis remains unclear. Herein, we determined the effects of a NO donor (sodium nitroprusside; SNP) and a NO-independent sGC activator (BAY 41-2272) in the growth and migration of rat aortic endothelial cells (RAEC). RAEC lack enzymatically active sGC as suggested by their inability to accumulate cGMP upon exposure to SNP. However, treatment of RAEC with SNP promoted a modest increase in their proliferation and migration that was dependent on extracellular signal regulated kinase1/2 activation. Moreover, when RAEC were exposed to vascular endothelial growth factor we observed an increase in migration that was inhibited by NO synthase, but not sGC, inhibition. Infection of cells with adenoviruses containing sGC greatly increased the efficacy of SNP as a mitogenic and migratory stimulus. We conclude that NO is capable of stimulating EC proliferation and mobility in the absence of sGC; however, increased intracellular levels of cGMP following sGC activation greatly amplify the angiogenic potential of NO.

Original languageEnglish (US)
Pages (from-to)1549-1554
Number of pages6
JournalLife Sciences
Volume81
Issue number21-22
DOIs
StatePublished - Nov 10 2007
Externally publishedYes

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Guanylate Cyclase
Nitric Oxide
Endothelial cells
Single Nucleotide Polymorphism
Rats
Endothelial Cells
Chemical activation
Nitric Oxide Donors
Nitroprusside
Soluble Guanylyl Cyclase
Adenoviridae
Nitric Oxide Synthase
Vascular Endothelial Growth Factor A
Blood Vessels
Cells
Growth
Infection

Keywords

  • cGMP
  • Guanylyl cyclase angiogenesis
  • Migration
  • Nitric oxide

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

cGMP-dependent and -independent angiogenesis-related properties of nitric oxide. / Pyriochou, Anastasia; Vassilakopoulos, Theodoros; Zhou, Zongmin; Papapetropoulos, Andreas.

In: Life Sciences, Vol. 81, No. 21-22, 10.11.2007, p. 1549-1554.

Research output: Contribution to journalArticle

Pyriochou, Anastasia ; Vassilakopoulos, Theodoros ; Zhou, Zongmin ; Papapetropoulos, Andreas. / cGMP-dependent and -independent angiogenesis-related properties of nitric oxide. In: Life Sciences. 2007 ; Vol. 81, No. 21-22. pp. 1549-1554.
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