Challenge with ovalbumin antigen increases uterine and cervical contractile activity in sensitized guinea pigs

Egle Bytautiene, Yuri P. Vedernikov, William L. Maner, George Saade, Roberto Romero, Robert E. Garfield

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Objective: The aims of this study were to investigate the effects of ovalbumin challenge on uterine and cervical contractility, intrauterine pressure, and uterine electromyography activity in sensitized guinea pigs. Study Design: Guinea pigs were sensitized by injection of ovalbumin-aluminum hydroxide suspension. Control animals were injected with the aluminum hydroxide suspension only. On days 55-57 of pregnancy, longitudinal uterine and cervical strips from guinea pigs were prepared for isometric tension recording. Nonpregnant guinea pigs were outfitted with telemetric transducers to record intrauterine pressure and uterine electromyography. Results: Ovalbumin significantly increased contractility of uterine and cervical strips from sensitized versus nonsensitized animals. These effects were abolished by histamine H1 receptor antagonist in uterine strips and by histamine H1 receptor antagonist and a mast cell stabilizer in cervical strips from sensitized animals. Cyclooxygenase and 5-lipoxygenase inhibitors had no significant effect on the response to ovalbumin. Treatment with ovalbumin in vivo significantly increased intrauterine pressure and uterine electromyography activity in sensitized but not in nonsensitized, animals. Conclusion: Our findings indicate that type I hypersensitivity reactions may be important in mediating uterine contractility in pregnant and nonpregnant states.

Original languageEnglish (US)
JournalAmerican Journal of Obstetrics and Gynecology
Volume199
Issue number6
DOIs
StatePublished - Dec 2008

Fingerprint

Ovalbumin
Guinea Pigs
Antigens
Electromyography
Histamine H1 Antagonists
Aluminum Hydroxide
Pressure
Suspensions
Immediate Hypersensitivity
Lipoxygenase Inhibitors
Prostaglandin-Endoperoxide Synthases
Transducers
Mast Cells
Pregnancy
Injections

Keywords

  • cervix
  • contractility
  • guinea pig
  • type I hypersensitivity reaction
  • uterus

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

Challenge with ovalbumin antigen increases uterine and cervical contractile activity in sensitized guinea pigs. / Bytautiene, Egle; Vedernikov, Yuri P.; Maner, William L.; Saade, George; Romero, Roberto; Garfield, Robert E.

In: American Journal of Obstetrics and Gynecology, Vol. 199, No. 6, 12.2008.

Research output: Contribution to journalArticle

Bytautiene, Egle ; Vedernikov, Yuri P. ; Maner, William L. ; Saade, George ; Romero, Roberto ; Garfield, Robert E. / Challenge with ovalbumin antigen increases uterine and cervical contractile activity in sensitized guinea pigs. In: American Journal of Obstetrics and Gynecology. 2008 ; Vol. 199, No. 6.
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