Changes in calcitonin gene-related peptide immunoreactivity in the rat dorsal horn following electrical stimulation of the sciatic nerve

C. M. Klein, R. E. Coggeshall, S. M. Carlton, K. N. Westlund, L. S. Sorkin

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Immunoreactive staining for calcitonin gene-related peptide (CGRP) in the rat superficial dorsal horn (L4-L5 segments) was examined following electrical stimulation of the sciatic nerve. Electrical stimulation for twenty minutes at an intensity to elicit both A and C fiber volleys resulted in a significant decrease in CGRP immunoreactivity in the medial two-thirds of the dorsal horn on the stimulated side as compared to the non-stimulated side of the spinal cord. The change was most pronounced in the medial area of the dorsal horn, with a 54% decrease in immunostaining density compared to the non-stimulated side. In contrast, when the sciatic nerve was stimulated for the same period at an intensity that stimulates only A fibers, there was no significant difference in the density of immunoreactive staining in the dorsal horn between the non-stimulated and stimulated sides of the spinal cord. These results suggest that activity in a specific population of primary afferent fibers causes a dramatic depletion of CGRP in the dorsal horn.

Original languageEnglish (US)
Pages (from-to)149-154
Number of pages6
JournalNeuroscience Letters
Volume115
Issue number2-3
DOIs
StatePublished - Jul 31 1990

Fingerprint

Calcitonin Gene-Related Peptide
Sciatic Nerve
Electric Stimulation
Myelinated Nerve Fibers
Spinal Cord
Staining and Labeling
Unmyelinated Nerve Fibers
Spinal Cord Dorsal Horn
Population

Keywords

  • Calcitonin gene-related peptide
  • Dorsal horn
  • Peripheral nerve stimulation
  • Primary afferent
  • Rat
  • Spinal cord

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Changes in calcitonin gene-related peptide immunoreactivity in the rat dorsal horn following electrical stimulation of the sciatic nerve. / Klein, C. M.; Coggeshall, R. E.; Carlton, S. M.; Westlund, K. N.; Sorkin, L. S.

In: Neuroscience Letters, Vol. 115, No. 2-3, 31.07.1990, p. 149-154.

Research output: Contribution to journalArticle

Klein, C. M. ; Coggeshall, R. E. ; Carlton, S. M. ; Westlund, K. N. ; Sorkin, L. S. / Changes in calcitonin gene-related peptide immunoreactivity in the rat dorsal horn following electrical stimulation of the sciatic nerve. In: Neuroscience Letters. 1990 ; Vol. 115, No. 2-3. pp. 149-154.
@article{18f00e13acbe4688b2cdf6a98edc4d2b,
title = "Changes in calcitonin gene-related peptide immunoreactivity in the rat dorsal horn following electrical stimulation of the sciatic nerve",
abstract = "Immunoreactive staining for calcitonin gene-related peptide (CGRP) in the rat superficial dorsal horn (L4-L5 segments) was examined following electrical stimulation of the sciatic nerve. Electrical stimulation for twenty minutes at an intensity to elicit both A and C fiber volleys resulted in a significant decrease in CGRP immunoreactivity in the medial two-thirds of the dorsal horn on the stimulated side as compared to the non-stimulated side of the spinal cord. The change was most pronounced in the medial area of the dorsal horn, with a 54{\%} decrease in immunostaining density compared to the non-stimulated side. In contrast, when the sciatic nerve was stimulated for the same period at an intensity that stimulates only A fibers, there was no significant difference in the density of immunoreactive staining in the dorsal horn between the non-stimulated and stimulated sides of the spinal cord. These results suggest that activity in a specific population of primary afferent fibers causes a dramatic depletion of CGRP in the dorsal horn.",
keywords = "Calcitonin gene-related peptide, Dorsal horn, Peripheral nerve stimulation, Primary afferent, Rat, Spinal cord",
author = "Klein, {C. M.} and Coggeshall, {R. E.} and Carlton, {S. M.} and Westlund, {K. N.} and Sorkin, {L. S.}",
year = "1990",
month = "7",
day = "31",
doi = "10.1016/0304-3940(90)90446-G",
language = "English (US)",
volume = "115",
pages = "149--154",
journal = "Neuroscience Letters",
issn = "0304-3940",
publisher = "Elsevier Ireland Ltd",
number = "2-3",

}

TY - JOUR

T1 - Changes in calcitonin gene-related peptide immunoreactivity in the rat dorsal horn following electrical stimulation of the sciatic nerve

AU - Klein, C. M.

AU - Coggeshall, R. E.

AU - Carlton, S. M.

AU - Westlund, K. N.

AU - Sorkin, L. S.

PY - 1990/7/31

Y1 - 1990/7/31

N2 - Immunoreactive staining for calcitonin gene-related peptide (CGRP) in the rat superficial dorsal horn (L4-L5 segments) was examined following electrical stimulation of the sciatic nerve. Electrical stimulation for twenty minutes at an intensity to elicit both A and C fiber volleys resulted in a significant decrease in CGRP immunoreactivity in the medial two-thirds of the dorsal horn on the stimulated side as compared to the non-stimulated side of the spinal cord. The change was most pronounced in the medial area of the dorsal horn, with a 54% decrease in immunostaining density compared to the non-stimulated side. In contrast, when the sciatic nerve was stimulated for the same period at an intensity that stimulates only A fibers, there was no significant difference in the density of immunoreactive staining in the dorsal horn between the non-stimulated and stimulated sides of the spinal cord. These results suggest that activity in a specific population of primary afferent fibers causes a dramatic depletion of CGRP in the dorsal horn.

AB - Immunoreactive staining for calcitonin gene-related peptide (CGRP) in the rat superficial dorsal horn (L4-L5 segments) was examined following electrical stimulation of the sciatic nerve. Electrical stimulation for twenty minutes at an intensity to elicit both A and C fiber volleys resulted in a significant decrease in CGRP immunoreactivity in the medial two-thirds of the dorsal horn on the stimulated side as compared to the non-stimulated side of the spinal cord. The change was most pronounced in the medial area of the dorsal horn, with a 54% decrease in immunostaining density compared to the non-stimulated side. In contrast, when the sciatic nerve was stimulated for the same period at an intensity that stimulates only A fibers, there was no significant difference in the density of immunoreactive staining in the dorsal horn between the non-stimulated and stimulated sides of the spinal cord. These results suggest that activity in a specific population of primary afferent fibers causes a dramatic depletion of CGRP in the dorsal horn.

KW - Calcitonin gene-related peptide

KW - Dorsal horn

KW - Peripheral nerve stimulation

KW - Primary afferent

KW - Rat

KW - Spinal cord

UR - http://www.scopus.com/inward/record.url?scp=0025335083&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025335083&partnerID=8YFLogxK

U2 - 10.1016/0304-3940(90)90446-G

DO - 10.1016/0304-3940(90)90446-G

M3 - Article

C2 - 2234494

AN - SCOPUS:0025335083

VL - 115

SP - 149

EP - 154

JO - Neuroscience Letters

JF - Neuroscience Letters

SN - 0304-3940

IS - 2-3

ER -