Changes in calcitonin gene-related peptide immunoreactivity in the rat dorsal horn following electrical stimulation of the sciatic nerve

C. M. Klein, R. E. Coggeshall, S. M. Carlton, K. N. Westlund, L. S. Sorkin

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

Immunoreactive staining for calcitonin gene-related peptide (CGRP) in the rat superficial dorsal horn (L4-L5 segments) was examined following electrical stimulation of the sciatic nerve. Electrical stimulation for twenty minutes at an intensity to elicit both A and C fiber volleys resulted in a significant decrease in CGRP immunoreactivity in the medial two-thirds of the dorsal horn on the stimulated side as compared to the non-stimulated side of the spinal cord. The change was most pronounced in the medial area of the dorsal horn, with a 54% decrease in immunostaining density compared to the non-stimulated side. In contrast, when the sciatic nerve was stimulated for the same period at an intensity that stimulates only A fibers, there was no significant difference in the density of immunoreactive staining in the dorsal horn between the non-stimulated and stimulated sides of the spinal cord. These results suggest that activity in a specific population of primary afferent fibers causes a dramatic depletion of CGRP in the dorsal horn.

Original languageEnglish (US)
Pages (from-to)149-154
Number of pages6
JournalNeuroscience Letters
Volume115
Issue number2-3
DOIs
StatePublished - Jul 31 1990

Keywords

  • Calcitonin gene-related peptide
  • Dorsal horn
  • Peripheral nerve stimulation
  • Primary afferent
  • Rat
  • Spinal cord

ASJC Scopus subject areas

  • Neuroscience(all)

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