Changes in mitochondrial functionality and calcium uptake in hypertensive rats as a function of age

E. Calderón-Cortés; C. Cortés-Rojo; M. Clemente-Guerrero; S. Manzo-Ávalos; R. Villalobos-Molina; I. Boldogh; A. Saavedra-Molina

doi:10.1016/j.mito.2008.04.005

Changes in mitochondrial functionality and calcium uptake in hypertensive rats as a function of age

E. Calderón-Cortés, C. Cortés-Rojo, M. Clemente-Guerrero, S. Manzo-Ávalos, R. Villalobos-Molina, I. Boldogh, A. Saavedra-Molina

Microbiology And Immunology

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

We studied whether mitochondrial functions and Ca²⁺ metabolism were altered in Wistar Kyoto normotensive (WKY) and spontaneous hypertensive rats (SHR). Ca²⁺ uptake was decreased in SHR compared to WKY rats. Accumulation of Ca²⁺ was more efficient in WKY than in SHR rats. mΔΨ was lower in SHR compared to WKY rats. Basal complex IV activity was higher in SHR than WKY rats, whereas basal l-citrulline production, an indicator of nitric oxide synthesis, was decreased in SHR and dependent on Ca²⁺ concentration (p < 0.05). Impact of Ca²⁺ was counteracted by EGTA. These data show an age-dependent decreased mitochondrial functions in brain mitochondria during hypertension.

Original language	English (US)
Pages (from-to)	262-272
Number of pages	11
Journal	Mitochondrion
Volume	8
Issue number	3
DOIs	https://doi.org/10.1016/j.mito.2008.04.005
State	Published - Jun 2008

Keywords

Aging
Brain mitochondria
Calcium uptake
Cytochrome c oxidase
Hypertension
Nitric oxide

ASJC Scopus subject areas

Molecular Medicine
Molecular Biology
Cell Biology

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10.1016/j.mito.2008.04.005

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title = "Changes in mitochondrial functionality and calcium uptake in hypertensive rats as a function of age",

abstract = "We studied whether mitochondrial functions and Ca2+ metabolism were altered in Wistar Kyoto normotensive (WKY) and spontaneous hypertensive rats (SHR). Ca2+ uptake was decreased in SHR compared to WKY rats. Accumulation of Ca2+ was more efficient in WKY than in SHR rats. mΔΨ was lower in SHR compared to WKY rats. Basal complex IV activity was higher in SHR than WKY rats, whereas basal l-citrulline production, an indicator of nitric oxide synthesis, was decreased in SHR and dependent on Ca2+ concentration (p < 0.05). Impact of Ca2+ was counteracted by EGTA. These data show an age-dependent decreased mitochondrial functions in brain mitochondria during hypertension.",

keywords = "Aging, Brain mitochondria, Calcium uptake, Cytochrome c oxidase, Hypertension, Nitric oxide",

author = "E. Calder{\'o}n-Cort{\'e}s and C. Cort{\'e}s-Rojo and M. Clemente-Guerrero and S. Manzo-{\'A}valos and R. Villalobos-Molina and I. Boldogh and A. Saavedra-Molina",

note = "Funding Information: The authors appreciate the partial financial support of CONACYT (43705, ASM and 47481, RVM), Fondos Mixtos CONACYT-Gobierno Estado Michoac{\'a}n (64277, ASM; 64308, SMA), COECYT (CB0702182_2, ASM) and CIC-UMSNH (2.16, ASM) grants. This work was also supported by P01 AG 021830 (IB) from the U.S NIA. ECC and CCR are CONACYT fellows.",

year = "2008",

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doi = "10.1016/j.mito.2008.04.005",

language = "English (US)",

volume = "8",

pages = "262--272",

journal = "Mitochondrion",

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AU - Calderón-Cortés, E.

AU - Cortés-Rojo, C.

AU - Clemente-Guerrero, M.

AU - Manzo-Ávalos, S.

AU - Villalobos-Molina, R.

AU - Boldogh, I.

AU - Saavedra-Molina, A.

N1 - Funding Information: The authors appreciate the partial financial support of CONACYT (43705, ASM and 47481, RVM), Fondos Mixtos CONACYT-Gobierno Estado Michoacán (64277, ASM; 64308, SMA), COECYT (CB0702182_2, ASM) and CIC-UMSNH (2.16, ASM) grants. This work was also supported by P01 AG 021830 (IB) from the U.S NIA. ECC and CCR are CONACYT fellows.

PY - 2008/6

Y1 - 2008/6

N2 - We studied whether mitochondrial functions and Ca2+ metabolism were altered in Wistar Kyoto normotensive (WKY) and spontaneous hypertensive rats (SHR). Ca2+ uptake was decreased in SHR compared to WKY rats. Accumulation of Ca2+ was more efficient in WKY than in SHR rats. mΔΨ was lower in SHR compared to WKY rats. Basal complex IV activity was higher in SHR than WKY rats, whereas basal l-citrulline production, an indicator of nitric oxide synthesis, was decreased in SHR and dependent on Ca2+ concentration (p < 0.05). Impact of Ca2+ was counteracted by EGTA. These data show an age-dependent decreased mitochondrial functions in brain mitochondria during hypertension.

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KW - Aging

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KW - Cytochrome c oxidase

KW - Hypertension

KW - Nitric oxide

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Changes in mitochondrial functionality and calcium uptake in hypertensive rats as a function of age

Abstract

Keywords

ASJC Scopus subject areas

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