Changes in mRNA expression of MMP-2 in the Müllerian duct of chicken embryo

Yonju Ha, Akira Tsukada, Noboru Saito, Kiyoshi Shimada

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Although asymmetric development of the ovary and the oviduct is a unique characteristic in birds, the mechanism of asymmetric development still remains unclear. Recently, degradation of extracellular matrix has been suggested as an important factor related to the regression of the Müllerian duct in mammals. The present study was conducted to examine a possible role of metalloproteinase-2 (MMP-2) in the regression of the right Müllerian duct in the developing chicken embryo. Morphological changes in the Müllerian ducts were studied on day 15 of incubation and mRNA expresseion of MMP-2 was studied on days 12, 15, and 18 of incubation. Morphological observation demonstrated the disappearance of basement membrane in the right Müllerian duct which undergoes the regression. RT-PCR analysis showed that MMP-2 mRNA expression of the right Müllerian duct increased on days 15 and 18 of incubation coincidently with the time of regression. In the right Müllerian duct, regression was prevented by diethylstilbestrol treatment on day 4 of incubation and a coincident decrease in MMP-2 expression was observed when compared to the control group. These results suggest that MMP-2 may be involved in the regression of the right Müllerian duct in the female embryos of the chicken.

Original languageEnglish (US)
Pages (from-to)131-136
Number of pages6
JournalGeneral and Comparative Endocrinology
Volume139
Issue number2
DOIs
StatePublished - Nov 2004
Externally publishedYes

Fingerprint

gelatinase A
Matrix Metalloproteinases
Chickens
embryo (animal)
Embryonic Structures
chickens
Messenger RNA
Diethylstilbestrol
Oviducts
Matrix Metalloproteinase 2
diethylstilbestrol
Basement Membrane
Birds
Extracellular Matrix
basement membrane
metalloproteinases
Mammals
Ovary
oviducts
extracellular matrix

Keywords

  • Chicken
  • Diethylstilbestrol
  • Embryo
  • Extracellular matrix
  • M̈llerian duct
  • Matrix metalloproteinase-2

ASJC Scopus subject areas

  • Endocrinology

Cite this

Changes in mRNA expression of MMP-2 in the Müllerian duct of chicken embryo. / Ha, Yonju; Tsukada, Akira; Saito, Noboru; Shimada, Kiyoshi.

In: General and Comparative Endocrinology, Vol. 139, No. 2, 11.2004, p. 131-136.

Research output: Contribution to journalArticle

Ha, Yonju ; Tsukada, Akira ; Saito, Noboru ; Shimada, Kiyoshi. / Changes in mRNA expression of MMP-2 in the Müllerian duct of chicken embryo. In: General and Comparative Endocrinology. 2004 ; Vol. 139, No. 2. pp. 131-136.
@article{46f1d39f0ac340c69d5275d70ca2865a,
title = "Changes in mRNA expression of MMP-2 in the M{\"u}llerian duct of chicken embryo",
abstract = "Although asymmetric development of the ovary and the oviduct is a unique characteristic in birds, the mechanism of asymmetric development still remains unclear. Recently, degradation of extracellular matrix has been suggested as an important factor related to the regression of the M{\"u}llerian duct in mammals. The present study was conducted to examine a possible role of metalloproteinase-2 (MMP-2) in the regression of the right M{\"u}llerian duct in the developing chicken embryo. Morphological changes in the M{\"u}llerian ducts were studied on day 15 of incubation and mRNA expresseion of MMP-2 was studied on days 12, 15, and 18 of incubation. Morphological observation demonstrated the disappearance of basement membrane in the right M{\"u}llerian duct which undergoes the regression. RT-PCR analysis showed that MMP-2 mRNA expression of the right M{\"u}llerian duct increased on days 15 and 18 of incubation coincidently with the time of regression. In the right M{\"u}llerian duct, regression was prevented by diethylstilbestrol treatment on day 4 of incubation and a coincident decrease in MMP-2 expression was observed when compared to the control group. These results suggest that MMP-2 may be involved in the regression of the right M{\"u}llerian duct in the female embryos of the chicken.",
keywords = "Chicken, Diethylstilbestrol, Embryo, Extracellular matrix, M̈llerian duct, Matrix metalloproteinase-2",
author = "Yonju Ha and Akira Tsukada and Noboru Saito and Kiyoshi Shimada",
year = "2004",
month = "11",
doi = "10.1016/j.ygcen.2004.08.003",
language = "English (US)",
volume = "139",
pages = "131--136",
journal = "General and Comparative Endocrinology",
issn = "0016-6480",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Changes in mRNA expression of MMP-2 in the Müllerian duct of chicken embryo

AU - Ha, Yonju

AU - Tsukada, Akira

AU - Saito, Noboru

AU - Shimada, Kiyoshi

PY - 2004/11

Y1 - 2004/11

N2 - Although asymmetric development of the ovary and the oviduct is a unique characteristic in birds, the mechanism of asymmetric development still remains unclear. Recently, degradation of extracellular matrix has been suggested as an important factor related to the regression of the Müllerian duct in mammals. The present study was conducted to examine a possible role of metalloproteinase-2 (MMP-2) in the regression of the right Müllerian duct in the developing chicken embryo. Morphological changes in the Müllerian ducts were studied on day 15 of incubation and mRNA expresseion of MMP-2 was studied on days 12, 15, and 18 of incubation. Morphological observation demonstrated the disappearance of basement membrane in the right Müllerian duct which undergoes the regression. RT-PCR analysis showed that MMP-2 mRNA expression of the right Müllerian duct increased on days 15 and 18 of incubation coincidently with the time of regression. In the right Müllerian duct, regression was prevented by diethylstilbestrol treatment on day 4 of incubation and a coincident decrease in MMP-2 expression was observed when compared to the control group. These results suggest that MMP-2 may be involved in the regression of the right Müllerian duct in the female embryos of the chicken.

AB - Although asymmetric development of the ovary and the oviduct is a unique characteristic in birds, the mechanism of asymmetric development still remains unclear. Recently, degradation of extracellular matrix has been suggested as an important factor related to the regression of the Müllerian duct in mammals. The present study was conducted to examine a possible role of metalloproteinase-2 (MMP-2) in the regression of the right Müllerian duct in the developing chicken embryo. Morphological changes in the Müllerian ducts were studied on day 15 of incubation and mRNA expresseion of MMP-2 was studied on days 12, 15, and 18 of incubation. Morphological observation demonstrated the disappearance of basement membrane in the right Müllerian duct which undergoes the regression. RT-PCR analysis showed that MMP-2 mRNA expression of the right Müllerian duct increased on days 15 and 18 of incubation coincidently with the time of regression. In the right Müllerian duct, regression was prevented by diethylstilbestrol treatment on day 4 of incubation and a coincident decrease in MMP-2 expression was observed when compared to the control group. These results suggest that MMP-2 may be involved in the regression of the right Müllerian duct in the female embryos of the chicken.

KW - Chicken

KW - Diethylstilbestrol

KW - Embryo

KW - Extracellular matrix

KW - M̈llerian duct

KW - Matrix metalloproteinase-2

UR - http://www.scopus.com/inward/record.url?scp=7044240926&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=7044240926&partnerID=8YFLogxK

U2 - 10.1016/j.ygcen.2004.08.003

DO - 10.1016/j.ygcen.2004.08.003

M3 - Article

VL - 139

SP - 131

EP - 136

JO - General and Comparative Endocrinology

JF - General and Comparative Endocrinology

SN - 0016-6480

IS - 2

ER -