Changes in Proteome Profile of Peripheral Blood Mononuclear Cells in Chronic Chagas Disease

Nisha Garg, Kizhake V. Soman, Maria P. Zago, Sue Jie Koo, Heidi Spratt, Susan Stafford, Zinzi N. Blell, Shivali Gupta, Julio Nuñez Burgos, Natalia Barrientos, Allan R. Brasier, John E. Wiktorowicz

Research output: Contribution to journalArticle

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Abstract

Trypanosoma cruzi (Tc) infection causes chagasic cardiomyopathy; however, why 30–40% of the patients develop clinical disease is not known. To discover the pathomechanisms in disease progression, we obtained the proteome signature of peripheral blood mononuclear cells (PBMCs) of normal healthy controls (N/H, n = 30) and subjects that were seropositive for Tc-specific antibodies, but were clinically asymptomatic (C/A, n = 25) or clinically symptomatic (C/S, n = 28) with cardiac involvement and left ventricular dysfunction. Protein samples were labeled with BODIPY FL-maleimide (dynamic range: > 4 orders of magnitude, detection limit: 5 f-mol) and resolved by two-dimensional gel electrophoresis (2D-GE). After normalizing the gel images, protein spots that exhibited differential abundance in any of the two groups were analyzed by mass spectrometry, and searched against UniProt human database for protein identification. We found 213 and 199 protein spots (fold change: |≥ 1.5|, p<0.05) were differentially abundant in C/A and C/S individuals, respectively, with respect to N/H controls. Ingenuity Pathway Analysis (IPA) of PBMCs proteome dataset identified an increase in disorganization of cytoskeletal assembly and recruitment/activation and migration of immune cells in all chagasic subjects, though the invasion capacity of cells was decreased in C/S individuals. IPA predicted with high probability a decline in cell survival and free radical scavenging capacity in C/S (but not C/A) subjects. The MYC/SP1 transcription factors that regulate hypoxia and oxidative/inflammatory stress were predicted to be key targets in the context of control of Chagas disease severity. Further, MARS-modeling identified a panel of proteins that had >93% prediction success in classifying infected individuals with no disease and those with cardiac involvement and LV dysfunction. In conclusion, we have identified molecular pathways and a panel of proteins that could aid in detecting seropositive individuals at risk of developing cardiomyopathy.

Original languageEnglish (US)
Article numbere0004490
JournalPLoS Neglected Tropical Diseases
Volume10
Issue number2
DOIs
StatePublished - Feb 26 2016

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Chagas Disease
Proteome
Blood Cells
Chronic Disease
Trypanosoma cruzi
Cardiomyopathies
Proteins
Protein Databases
Electrophoresis, Gel, Two-Dimensional
Left Ventricular Dysfunction
Disease Progression
Limit of Detection
Mass Spectrometry
Gels
Antibodies
Infection

ASJC Scopus subject areas

  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Changes in Proteome Profile of Peripheral Blood Mononuclear Cells in Chronic Chagas Disease. / Garg, Nisha; Soman, Kizhake V.; Zago, Maria P.; Koo, Sue Jie; Spratt, Heidi; Stafford, Susan; Blell, Zinzi N.; Gupta, Shivali; Nuñez Burgos, Julio; Barrientos, Natalia; Brasier, Allan R.; Wiktorowicz, John E.

In: PLoS Neglected Tropical Diseases, Vol. 10, No. 2, e0004490, 26.02.2016.

Research output: Contribution to journalArticle

Garg, N, Soman, KV, Zago, MP, Koo, SJ, Spratt, H, Stafford, S, Blell, ZN, Gupta, S, Nuñez Burgos, J, Barrientos, N, Brasier, AR & Wiktorowicz, JE 2016, 'Changes in Proteome Profile of Peripheral Blood Mononuclear Cells in Chronic Chagas Disease', PLoS Neglected Tropical Diseases, vol. 10, no. 2, e0004490. https://doi.org/10.1371/journal.pntd.0004490
Garg, Nisha ; Soman, Kizhake V. ; Zago, Maria P. ; Koo, Sue Jie ; Spratt, Heidi ; Stafford, Susan ; Blell, Zinzi N. ; Gupta, Shivali ; Nuñez Burgos, Julio ; Barrientos, Natalia ; Brasier, Allan R. ; Wiktorowicz, John E. / Changes in Proteome Profile of Peripheral Blood Mononuclear Cells in Chronic Chagas Disease. In: PLoS Neglected Tropical Diseases. 2016 ; Vol. 10, No. 2.
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