Chapare virus, a newly discovered arenavirus isolated from a fatal hemorrhagic fever case in Bolivia

Simon Delgado, Bobbie R. Erickson, Roberto Agudo, Patrick J. Blair, Efrain Vallejo, César G. Albariño, Jorge Vargas, James A. Comer, Pierre E. Rollin, Thomas Ksiazek, James G. Olson, Stuart T. Nichol

Research output: Contribution to journalArticle

125 Citations (Scopus)

Abstract

A small focus of hemorrhagic fever (HF) cases occurred near Cochabamba, Bolivia, in December 2003 and January 2004. Specimens were available from only one fatal case, which had a clinical course that included fever, headache, arthralgia, myalgia, and vomiting with subsequent deterioration and multiple hemorrhagic signs. A non-cytopathic virus was isolated from two of the patient serum samples, and identified as an arenavirus by IFA staining with a rabbit polyvalent antiserum raised against South American arenaviruses known to be associated with HF (Guanarito, Machupo, and Sabiá). RT-PCR analysis and subsequent analysis of the complete virus S and L RNA segment sequences identified the virus as a member of the New World Clade B arenaviruses, which includes all the pathogenic South American arenaviruses. The virus was shown to be most closely related to Sabiá virus, but with 26% and 30% nucleotide difference in the S and L segments, and 26%, 28%, 15% and 22% amino acid differences for the L, Z, N, and GP proteins, respectively, indicating the virus represents a newly discovered arenavirus, for which we propose the name Chapare virus. In conclusion, two different arenaviruses, Machupo and Chapare, can be associated with severe HF cases in Bolivia.

Original languageEnglish (US)
Article numbere1000047
JournalPLoS Pathogens
Volume4
Issue number4
DOIs
StatePublished - Apr 2008
Externally publishedYes

Fingerprint

New World Arenaviruses
Arenavirus
Bolivia
Fever
Viruses
Myalgia
Arthralgia
Names
Vomiting
Headache
Immune Sera
Nucleotides
Staining and Labeling
Rabbits
Amino Acids
Polymerase Chain Reaction
Serum

ASJC Scopus subject areas

  • Microbiology
  • Parasitology
  • Virology
  • Immunology
  • Genetics
  • Molecular Biology

Cite this

Delgado, S., Erickson, B. R., Agudo, R., Blair, P. J., Vallejo, E., Albariño, C. G., ... Nichol, S. T. (2008). Chapare virus, a newly discovered arenavirus isolated from a fatal hemorrhagic fever case in Bolivia. PLoS Pathogens, 4(4), [e1000047]. https://doi.org/10.1371/journal.ppat.1000047

Chapare virus, a newly discovered arenavirus isolated from a fatal hemorrhagic fever case in Bolivia. / Delgado, Simon; Erickson, Bobbie R.; Agudo, Roberto; Blair, Patrick J.; Vallejo, Efrain; Albariño, César G.; Vargas, Jorge; Comer, James A.; Rollin, Pierre E.; Ksiazek, Thomas; Olson, James G.; Nichol, Stuart T.

In: PLoS Pathogens, Vol. 4, No. 4, e1000047, 04.2008.

Research output: Contribution to journalArticle

Delgado, S, Erickson, BR, Agudo, R, Blair, PJ, Vallejo, E, Albariño, CG, Vargas, J, Comer, JA, Rollin, PE, Ksiazek, T, Olson, JG & Nichol, ST 2008, 'Chapare virus, a newly discovered arenavirus isolated from a fatal hemorrhagic fever case in Bolivia', PLoS Pathogens, vol. 4, no. 4, e1000047. https://doi.org/10.1371/journal.ppat.1000047
Delgado, Simon ; Erickson, Bobbie R. ; Agudo, Roberto ; Blair, Patrick J. ; Vallejo, Efrain ; Albariño, César G. ; Vargas, Jorge ; Comer, James A. ; Rollin, Pierre E. ; Ksiazek, Thomas ; Olson, James G. ; Nichol, Stuart T. / Chapare virus, a newly discovered arenavirus isolated from a fatal hemorrhagic fever case in Bolivia. In: PLoS Pathogens. 2008 ; Vol. 4, No. 4.
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