Characterisation of Caenorhabditis elegans sperm transcriptome and proteome

Xuan Ma, Yingjie Zhu, Chunfang Li, Peng Xue, Yanmei Zhao, Shilin Chen, Fuquan Yang, Long Miao

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Background:Although sperm is transcriptionally and translationally quiescent, complex populations of RNAs, including mRNAs and non-coding RNAs, exist in sperm. Previous microarray analysis of germ cell mutants identified hundreds of sperm genes in Caenorhabditis elegans. To take a more comprehensive view on C. elegans sperm genes, here, we isolate highly pure sperm cells and employ high-throughput technologies to obtain sperm transcriptome and proteome.Results:First, sperm transcriptome consists of considerable amounts of non-coding RNAs, many of which have not been annotated and may play functional roles during spermatogenesis. Second, apart from kinases/phosphatases as previously reported, ion binding proteins are also enriched in sperm, underlying the crucial roles of intracellular ions in post-translational regulation in sperm. Third, while the majority of sperm genes/proteins have low abundance, a small number of sperm genes/proteins are hugely enriched in sperm, implying that sperm only rely on a small set of proteins for post-translational regulation. Lastly, by extensive RNAi screening of sperm enriched genes, we identified a few genes that control fertility. Our further analysis reveals a tight correlation between sperm transcriptome and sperm small RNAome, suggesting that the endogenous siRNAs strongly repress sperm genes. This leads to an idea that the inefficient RNAi screening of sperm genes, a phenomenon currently with unknown causes, might result from the competition between the endogenous RNAi pathway and the exogenous RNAi pathway.Conclusions:Together, the obtained sperm transcriptome and proteome serve as valuable resources to systematically study spermatogenesis in C. elegans.

Original languageEnglish (US)
Article number168
JournalBMC Genomics
Volume15
Issue number1
DOIs
StatePublished - Feb 28 2014
Externally publishedYes

Fingerprint

Caenorhabditis elegans
Proteome
Transcriptome
Spermatozoa
RNA Interference
Genes
Untranslated RNA
Spermatogenesis
Ions
Proteins
Sperm Count
Microarray Analysis
Contraception

Keywords

  • C. elegans
  • Proteome
  • Sperm
  • Transcriptome

ASJC Scopus subject areas

  • Biotechnology
  • Genetics

Cite this

Characterisation of Caenorhabditis elegans sperm transcriptome and proteome. / Ma, Xuan; Zhu, Yingjie; Li, Chunfang; Xue, Peng; Zhao, Yanmei; Chen, Shilin; Yang, Fuquan; Miao, Long.

In: BMC Genomics, Vol. 15, No. 1, 168, 28.02.2014.

Research output: Contribution to journalArticle

Ma, X, Zhu, Y, Li, C, Xue, P, Zhao, Y, Chen, S, Yang, F & Miao, L 2014, 'Characterisation of Caenorhabditis elegans sperm transcriptome and proteome', BMC Genomics, vol. 15, no. 1, 168. https://doi.org/10.1186/1471-2164-15-168
Ma, Xuan ; Zhu, Yingjie ; Li, Chunfang ; Xue, Peng ; Zhao, Yanmei ; Chen, Shilin ; Yang, Fuquan ; Miao, Long. / Characterisation of Caenorhabditis elegans sperm transcriptome and proteome. In: BMC Genomics. 2014 ; Vol. 15, No. 1.
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