Characteristics of Trophoblasts in Long-Term Culture

T. D. Kolokol’tsova, I. N. Saburina, Tatiana Nanovskaya, S. L. Patrikeeva, D. I. Vernikovskaya, I. M. Zurina, A. A. Gorkun, N. V. Kosheleva, R. A. Poltavtseva, G. T. Sukhikh

Research output: Contribution to journalArticle

Abstract

We analyzed more than 40 cytotrophoblast cultures derived from cell islets that grew from trypsinized tissue fragments of placental microvilli. Phenotypic variability of trophoblasts was demonstrated. Changes in trophoblast morphology from epithelium-like or oval cells to bipolar and spindle-shaped or twisted and then to mesenchymal-like cells as well as intensive expression of cytokeratin-7 and vimentin attested to epithelial-mesenchymal transition of trophoblasts during in vitro culturing. Analysis of the expression of specific markers in long-term trophoblast culture (≥7 passages) revealed the possibility of culture contamination with other non-trophoblast cells including fibroblasts. High risk of trophoblast culture contamination with rapidly growing cells necessitates regular control of the cultures used in fundamental studies. Our experiments confirmed the possibility of long-term culturing of cells maintaining trophoblast properties. The identity and purity of 4 trophoblast cultures free from contamination and retaining the properties of pure culture during long-term (>10 passages) culturing in vitro were confirmed.

Original languageEnglish (US)
Pages (from-to)259-265
Number of pages7
JournalBulletin of Experimental Biology and Medicine
Volume164
Issue number2
DOIs
StatePublished - Dec 1 2017

Keywords

  • cell culture
  • cytokeratin
  • trophoblast
  • trophoblast morphology
  • vimentin

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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  • Cite this

    Kolokol’tsova, T. D., Saburina, I. N., Nanovskaya, T., Patrikeeva, S. L., Vernikovskaya, D. I., Zurina, I. M., Gorkun, A. A., Kosheleva, N. V., Poltavtseva, R. A., & Sukhikh, G. T. (2017). Characteristics of Trophoblasts in Long-Term Culture. Bulletin of Experimental Biology and Medicine, 164(2), 259-265. https://doi.org/10.1007/s10517-017-3969-6